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Default Proton-clouds to measure long-range contacts between non-exchangeable sidechain protons in solid-state NMR.

Proton-clouds to measure long-range contacts between non-exchangeable sidechain protons in solid-state NMR.

Related Articles Proton-clouds to measure long-range contacts between non-exchangeable sidechain protons in solid-state NMR.

J Am Chem Soc. 2014 Jan 27;

Authors: Sinnige T, Daniels M, Baldus M, Weingarth M

Abstract
We show that selective labeling of proteins with protonated amino acids embedded in a perdeuterated matrix, dubbed 'proton clouds', provides general access to long-range contacts between non-exchangeable sidechain protons in proton-detected solid-state NMR, which is important to study protein tertiary structure. Proton-cloud labeling significantly improves spectral resolution by simultaneously reducing proton linewidth and spectral crowding despite a high local proton density in clouds. The approach is amenable to almost all canonical amino acids. Our method is demonstrated on ubiquitin and the ?-barrel membrane protein BamA.


PMID: 24467345 [PubMed - as supplied by publisher]



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