[NMR paper] Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy
Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy
High-resolution structural studies of proteins and protein complexes in a native eukaryotic environment present a challenge to structural biology. In-cell NMR can characterize atomic resolution structures but requires high concentrations of labeled proteins in intact cells. Most exogenous delivery techniques are limited to specific cell types or are too destructive to preserve cellular physiology. The feasibility of microfluidics transfection or volume exchange for convective transfer, VECT,...
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05-14-2022 03:47 AM
[NMR paper] Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell...
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03-30-2021 08:29 AM
[NMR paper] In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation.
In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation.
In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation.
Acc Chem Res. 2018 Jun 05;:
Authors: Luchinat E, Banci L
Abstract
Cellular structural biology methods are needed to characterize biological processes at atomic resolution in the physiological environment of the cell. Toward this goal, solution in-cell NMR is a powerful approach because it provides...
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06-06-2018 01:42 PM
[NMR paper] Sequential protein expression and selective labeling for in-cell NMR in human cells.
Sequential protein expression and selective labeling for in-cell NMR in human cells.
Related Articles Sequential protein expression and selective labeling for in-cell NMR in human cells.
Biochim Biophys Acta. 2015 Dec 23;
Authors: Luchinat E, Secci E, Cencetti F, Bruni P
Abstract
BACKGROUND: In-cell NMR is a powerful technique to investigate proteins in living human cells at atomic resolution. Ideally, when studying functional processes involving protein-protein interactions by NMR, only one partner should be isotopically...
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Sequential protein expression and selective labeling for in-cell NMR in human cells
Sequential protein expression and selective labeling for in-cell NMR in human cells
Publication date: Available online 23 December 2015
Source:Biochimica et Biophysica Acta (BBA) - General Subjects</br>
Author(s): Enrico Luchinat, Erica Secci, Francesca Cencetti, Paola Bruni</br>
Background In-cell NMR is a powerful technique to investigate proteins in living human cells at atomic resolution. Ideally, when studying functional processes involving protein-protein interactions by NMR, only one partner should be isotopically labeled. Here we show that...
Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression
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