It is a unique trait of the NMR method for protein structure determination that a description of the polypeptide secondary structure can be obtained at an early stage and quite independently of the complete structure calculation. In this paper the procedures used for secondary structure determination are reviewed and placed in perspective relative to the other steps in a complete three-dimensional structure determination. As an illustration the identification of the regular secondary structure elements in human cyclophilin is described.
Application of SAIL phenylalanine and tyrosine with alternative isotope-labeling patterns for protein structure determination
Application of SAIL phenylalanine and tyrosine with alternative isotope-labeling patterns for protein structure determination
Abstract The extensive collection of NOE constraint data involving the aromatic ring signals is essential for accurate protein structure determination, although it is often hampered in practice by the pervasive signal overlapping and tight spin couplings for aromatic rings. We have prepared various types of stereo-array isotope labeled phenylalanines (ε- and ζ-SAIL Phe) and tyrosine (ε-SAIL Tyr) to overcome these problems (Torizawa et al. 2005), and proven...
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[NMR paper] Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.
Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.
Related Articles Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha.
Protein Sci. 2005 Sep;14(9):2421-8
Authors: Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ
Coiled-coil motifs play essential roles in protein assembly and molecular recognition, and are therefore the targets of many ongoing...
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[NMR paper] Structure of recombinant human parathyroid hormone in solution using multidimensional
Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy.
Related Articles Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy.
Biol Chem Hoppe Seyler. 1996 Mar;377(3):175-86
Authors: Gronwald W, Schomburg D, Harder MP, Mayer H, Paulsen J, Wingender E, Wray V
The solution structure of human parathyroid hormone, in the form of recombinant prolyl-hPTH(1-84), has been investigated by multidimensional NMR spectroscopy under conditions (aqueous...
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[NMR paper] Secondary structure and zinc ligation of human recombinant short-form stromelysin by
Secondary structure and zinc ligation of human recombinant short-form stromelysin by multidimensional heteronuclear NMR.
Related Articles Secondary structure and zinc ligation of human recombinant short-form stromelysin by multidimensional heteronuclear NMR.
Biochemistry. 1993 Dec 7;32(48):13098-108
Authors: Gooley PR, Johnson BA, Marcy AI, Cuca GC, Salowe SP, Hagmann WK, Esser CK, Springer JP
Stromelysin-1, a member of the matrix metalloendoprotease family, is a zinc protease involved in the degradation of connective tissue in the...
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[NMR paper] Secondary structure and protein folding of recombinant chloroplastic thioredoxin Ch2
Secondary structure and protein folding of recombinant chloroplastic thioredoxin Ch2 from the green alga Chlamydomonas reinhardtii as determined by 1H NMR.
Related Articles Secondary structure and protein folding of recombinant chloroplastic thioredoxin Ch2 from the green alga Chlamydomonas reinhardtii as determined by 1H NMR.
J Biochem. 1993 Sep;114(3):421-31
Authors: Lancelin JM, Stein M, Jacquot JP
The recombinant form of the chloroplastic thioredoxin Ch2 from the green alga Chlamydomonas reinhardtii that preferentially activates the NADP...
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[NMR paper] Determination of the structure of the nucleocapsid protein NCp7 from the human immuno
Determination of the structure of the nucleocapsid protein NCp7 from the human immunodeficiency virus type 1 by 1H NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Determination of the structure of the nucleocapsid protein NCp7 from the human immunodeficiency virus type 1 by 1H NMR.
EMBO J. 1992 Aug;11(8):3059-65
Authors: Morellet N, Jullian N, De Rocquigny H, Maigret B, Darlix JL, Roques BP
The retroviral gag nucleocapsid protein NCp7 (72 amino acids)...
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[NMR paper] Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-d
Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-digoxin antibody VL domain.
Related Articles Sequential 1H and 15N NMR assignments and secondary structure of a recombinant anti-digoxin antibody VL domain.
Biochemistry. 1992 Jun 2;31(21):5033-43
Authors: Constantine KL, Goldfarb V, Wittekind M, Anthony J, Ng SC, Mueller L
A uniformly 15N-labeled recombinant light-chain variable (VL) domain from the anti-digoxin antibody 26-10 has been investigated by heteronuclear two-dimensional (2D) and three-dimensional...
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[NMR paper] Protein secondary structure determination by NMR. Application with recombinant human
Protein secondary structure determination by NMR. Application with recombinant human cyclophilin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein secondary structure determination by NMR. Application with recombinant human cyclophilin.
FEBS Lett. 1991 Jul 22;285(2):237-47
Authors: Wüthrich K, Spitzfaden C, Memmert K, Widmer H, Wider G
It is a unique trait of the NMR method for protein structure determination that a description of the polypeptide secondary...
Protein secondary structure determination by NMR. Application with recombinant human
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