Protein residue linking in a single spectrum for magic-angle spinning NMR assignment
 

Protein residue linking in a single spectrum for magic-angle spinning NMR assignment

Abstract

Here we introduce a new pulse sequence for resonance assignment that halves the number of data sets required for sequential linking by directly correlating sequential amide resonances in a single diagonal-free spectrum. The method is demonstrated with both microcrystalline and sedimented deuterated proteins spinning at 60 and 111Â*kHz, and a fully protonated microcrystalline protein spinning at 111Â*kHz, with as little as 0.5Â*mg protein sample. We find that amide signals have a low chance of ambiguous linkage, which is further improved by linking in both forward and backward directions. The spectra obtained are amenable to automated resonance assignment using general-purpose software such as UNIO-MATCH.



Source: Journal of Biomolecular NMR