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Default Protein and lipid dynamics in photosynthetic thylakoid membranes investigated by in-situ solid-state NMR.

Protein and lipid dynamics in photosynthetic thylakoid membranes investigated by in-situ solid-state NMR.

Related Articles Protein and lipid dynamics in photosynthetic thylakoid membranes investigated by in-situ solid-state NMR.

Biochim Biophys Acta. 2016 Sep 11;

Authors: Chegeni FA, Perin G, Gupta KB, Simionato D, Morosinotto T, Pandit A

Abstract
Photosynthetic thylakoid membranes contain the protein machinery to convert sunlight in chemical energy and regulate this process in changing environmental conditions via interplay between lipid, protein and xanthophyll molecular constituents. This work addresses the molecular effects of zeaxanthin accumulation in thylakoids, which occurs in native systems under high light conditions through the conversion of the xanthophyll violaxanthin into zeaxanthin via the so called xanthophyll cycle. We applied biosynthetic isotope labeling and (13)C solid-state NMR spectroscopy to simultaneously probe the conformational dynamics of protein, lipid and xanthophyll constituents of thylakoids isolated from wild type (cw15) and npq2 mutant of the green alga Chlamydomonas reinhardtii, that accumulates zeaxanthin constitutively. Results show differential dynamics of wild type and npq2 thylakoids. Ordered-phase lipids have reduced mobility and mobile-phase lipids have enlarged dynamics in npq2 membranes, together spanning a broader dynamical range. The fraction of ordered lipids is much larger than the fraction of mobile lipids, which explains why zeaxanthin appears to cause overall reduction of thylakoid membrane fluidity. In addition to the ordered lipids, also the xanthophylls and a subset of protein sites in npq2 thylakoids have reduced conformational dynamics. Our work demonstrates the applicability of solid-state NMR spectroscopy for obtaining a microscopic picture of different membrane constituents simultaneously, inside native, heterogeneous membranes.


PMID: 27626974 [PubMed - as supplied by publisher]



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