Protein Electrostatics Investigated through Paramagnetic NMR for Nonpolar Groups
 

Protein Electrostatics Investigated through Paramagnetic NMR for Nonpolar Groups

Experimental validation of theoretical models for protein electrostatics remains rare. Recently, we have developed a paramagnetic NMR-based method for de novo determination of effective near-surface electrostatic potentials, which allows for straightforward examination of electrostatic models for biomolecules. In the current work, we expand this method and demonstrate that effective near-surface electrostatic potentials can readily be determined from ¹H paramagnetic relaxation enhancement (PRE)...

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