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Default A solution (17)O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase.

A solution (17)O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase.

Related Articles A solution (17)O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase.

Chem Commun (Camb). 2013 Jan 22;49(14):1449-51

Authors: Hanashima S, Fujiwara N, Matsumoto K, Iwasaki N, Zheng GQ, Torigoe H, Suzuki K, Taniguchi N, Yamaguchi Y

Abstract
Solution (17)O-NMR application to biological macromolecules is extremely limited. We describe here (17)O-NMR observation of the (17)O(2)-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective (17)O(2) oxidation. (17)O-NMR with the aid of (17)O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.


PMID: 23323266 [PubMed - in process]



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