BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 09-17-2008, 10:12 PM
Junior Member
 
Join Date: Sep 2008
Posts: 2
Points: 24, Level: 1
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Level up: 47%, 26 Points needed
Level up: 47% Level up: 47% Level up: 47%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 24
Downloads: 0
Uploads: 0
Default Prospects for lanthanides in structural biology by NMR

Prospects for lanthanides in structural biology by NMR
Gottfried Otting
Journal of Biomolecular NMR; 2008; 42(1); pp 1-9

Abstract:

The advent of different lanthanide-binding reagents has made site-specific labelling of proteins with paramagnetic lanthanides a viable proposition. This brings many powerful techniques originally established and demonstrated for paramagnetic metalloproteins into the mainstream of structural biology. The promise is that, by exploiting the long-range effects of paramagnetism, lanthanide labelling will allow the study of larger proteins and protein–ligand complexes with greater ease and accuracy than hitherto possible. In particular, lanthanide-induced pseudocontact shifts (PCS) provide powerful restraints and 3D structure determination using PCS as the only source of experimental restraints will probably be possible with data obtained from samples with different lanthanide-tagging sites. Cell-free protein synthesis is positioned to play an important role in this strategy, as an inexpensive source of selectively labelled protein samples and for easy site-specific incorporation of unnatural lanthanide-binding amino acids.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Postoctoral position in Structural Biology
Postoctoral position in Structural Biology UNIVERSITY OF OXFORD DEPARTMENT OF BIOCHEMISTRY Postdoctoral Research Associate in Structural Biology We are seeking to appoint a highly motivated Postdoctoral Research Associate in the laboratory of Dr. Ioannis Vakonakis for studies of proteins involved in centriole assembly. The successful candidate will
nmrlearner Job marketplace 0 01-27-2012 04:34 PM
Structural Biology Widget
Structural Biology Widget Structural Biology Knowledge Base Widget More...
nmrlearner General 0 04-15-2011 01:40 AM
[NMR900 blog] NMR paper in Nature Structural & Molecular Biology
NMR paper in Nature Structural & Molecular Biology L.A. Freiburger, O.M. Baettig, T. Sprules, A.M. Berghuis, K. Auclair, A.K. Mittermaier, "Competing allosteric mechanisms modulate substrate binding in a dimeric enzyme," Nature Structural & Molecular Biology (2011) online. http://dx.doi.org/10.1038/nsmb.1978 https://blogger.googleusercontent.com/tracker/8663203727601106205-794484937251380301?l=nmr900.blogspot.com Read complete story on NMR900 blog
nmrlearner News from NMR blogs 0 02-11-2011 08:31 AM
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new d... More...
nmrlearner Job marketplace 0 01-26-2011 04:21 AM
Structural biology faculty position at MCW
Just got info about the following position:
markber Job marketplace 0 09-25-2010 10:45 AM
[NMR paper] An account of NMR in structural biology.
An account of NMR in structural biology. Related Articles An account of NMR in structural biology. Nat Struct Biol. 1997 Oct;4 Suppl:841-4 Authors: Wagner G With the ability to determine atomic resolution structures of biological macromolecules in semi-physiological conditions, nuclear magnetic resonance spectroscopy (NMR) has become an eminent tool in structural biology. NMR provides a means for studying critical biological phenomena including protein structure, dynamics and folding as well as a practical approach to drug design.
nmrlearner Journal club 0 08-22-2010 05:08 PM
Postdoctoral positions in Biochemistry and Structural Biology
Postdoctoral positions in Biochemistry and Structural Biology Two postdoc positions are currently available in the laboratory of Dr. Jamil Saad at the University of Alabama at Birmingham (UAB). Our research program is aimed at understanding the precise molecular mechanism of how retroviral proteins interact with cellular factors during the virus replication cycle. We use a set of biochemical, biophysical and structural biology (mainly NMR and occasionally X-ray) tools to characterize key protein-protein and protein-lipid interactions critical for vi… More...
nmrlearner Job marketplace 0 08-19-2010 02:44 AM
Another lecture by Gregg Siegal - NMR and Structural Biology
Gregg Siegal (Leiden Institute of Chemistry) discusses the application of NMR to structural biology. http://metprot.lic.gorlaeus.net/siegal/teaching/struc_biol/NMR_and_Structural_Biology.ppt The website of the LIC's protein chemistry department can be found here: http://protchem.lic.gorlaeus.net/index.php?nav=home
Dermot Educational web pages 0 09-08-2008 11:46 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 05:59 PM.


Map