Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
 

Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells


Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid bilayers using oriented bilayers and magical angle spinning techniques. In particular, Boltzmann statistics REDOR and maximum entropy analysis of spinning side bands are used to analyse systems with multiple states of peptide or lipid molecules may co-exist. We propose that in future, rather than model membranes, structural studies in whole cells are feasible.
Graphical abstract

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Source: Journal of Magnetic Resonance