Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells
 

Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells


Abstract The preparation of stable isotope-labeled proteins is necessary for the application of a wide variety of NMR methods, to study the structures and dynamics of proteins and protein complexes. The E. coli expression system is generally used for the production of isotope-labeled proteins, because of the advantages of ease of handling, rapid growth, high-level protein production, and low cost for isotope-labeling. However, many eukaryotic proteins are not functionally expressed in E. coli, due to problems related to disulfide bond formation, post-translational modifications, and folding. In such cases, other expression systems are required for producing proteins for biomolecular NMR analyses. In this paper, we review the recent advances in expression systems for isotopically labeled heterologous proteins, utilizing non-E. coli prokaryotic and eukaryotic cells.

• Content Type Journal Article
• Pages 3-10
• DOI 10.1007/s10858-009-9377-0
• Authors
  • Hideo Takahashi, National Institute of Advanced Industrial Science and Technology (AIST) Biomedicinal Information Research Center (BIRC) Aomi, Koto-ku Tokyo 135-0064 Japan
  • Ichio Shimada, National Institute of Advanced Industrial Science and Technology (AIST) Biomedicinal Information Research Center (BIRC) Aomi, Koto-ku Tokyo 135-0064 Japan

• • Journal Journal of Biomolecular NMR
  • Online ISSN 1573-5001
  • Print ISSN 0925-2738
  • Journal Volume Volume 46
  • Journal Issue Volume 46, Number 1

Source: Journal of Biomolecular NMR