Production of isotope-labeled proteins in insect cells for NMR.
 

Production of isotope-labeled proteins in insect cells for NMR.

Related Articles Production of isotope-labeled proteins in insect cells for NMR.

J Biomol NMR. 2018 Apr 23;:

Authors: Franke B, Opitz C, Isogai S, Grahl A, Delgado L, Gossert AD, Grzesiek S

Abstract
Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.


PMID: 29687312 [PubMed - as supplied by publisher]



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