BioNMR

BioNMR (http://www.bionmr.com/forum/)
-   Journal club (http://www.bionmr.com/forum/journal-club-9/)
-   -   Production of isotope-labeled proteins in insect cells for NMR (http://www.bionmr.com/forum/journal-club-9/production-isotope-labeled-proteins-insect-cells-nmr-25954/)

nmrlearner 04-23-2018 05:00 PM

Production of isotope-labeled proteins in insect cells for NMR
 
Production of isotope-labeled proteins in insect cells for NMR

Abstract

Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.



Source: Journal of Biomolecular NMR


All times are GMT. The time now is 01:10 PM.

Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013