Concentration-dependent changes to diffusion and chemical shift of internal standard molecules in aqueous and micellar solutions
Concentration-dependent changes to diffusion and chemical shift of internal standard molecules in aqueous and micellar solutions
Abstract
Sodium 4,4-dimethyl-4-silapentane-1-sulfonate (DSS) is the most widely accepted internal standard for protein NMR studies in aqueous conditions. Since its introduction as a reference standard, however, concerns have been raised surrounding its propensity to interact with biological molecules through electrostatic and hydrophobic interactions. While DSS has been shown to interact with certain proteins, membrane...
[NMR paper] Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
FEBS Lett. 2014 Apr 3;
Authors: Sommer LA, Dames SA
...
[Question from NMRWiki Q&A forum] Concentration-dependent signal fine splitting
Concentration-dependent signal fine splitting
Hi,
I have synthesized coproporphyrin II tetramethyl ester and taken a 1H NMR in CDCl3:
http://illumina-chemie.de/upload/5_11600286385136157fa7971.png
Especially the peaks at 10.1 ppm, belonging to the CH groups connecting the pyrrole rings, show a strong concentration dependence. They should be two singlets, but in fact they are only singlets at very low concentrations and split up with increasing concentration:
nmrlearner
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03-06-2013 04:21 AM
[NMR paper] Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein.
Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein.
Biopolymers. 2012;98(1):59-66
Authors: Xue R, Liu Y, Zheng Y, Wu Y, Li X, Pei F, Ni J
Abstract
The occurrence of a highly conserved 11-mer repeating...
[NMR paper] Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS
Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS NMR using lanthanide ions.
Related Articles Probing membrane surfaces and the location of membrane-embedded peptides by (13)C MAS NMR using lanthanide ions.
J Magn Reson. 1999 Dec;141(2):335-9
Authors: Gröbner G, Glaubitz C, Watts A
A simple but efficient (13)C MAS NMR method is presented for the determination of the location of embedded molecules such as peptides relative to biological membrane surfaces by exploiting the interaction with paramagnetic...
Probing Membrane Protein Association Using Concentration-Dependent Number and Brightness
Linear Mode
