Solid-state NMR (ssNMR) spectroscopy facilitates the non-destructive characterization of structurally heterogeneous biomolecules in their native setting, e.g., comprising proteins, lipids and polysaccharides. Here we demonstrate the utility of high and ultra-high field 1H-detected fast MAS ssNMR spectroscopy, which exhibits increased sensitivity and spectral resolution to further elucidate the atomic-level composition and structural arrangement of the cell wall of Schizophyllum commune, a...
[NMR paper] Solid-state NMR of plant and fungal cell walls: A critical review.
Solid-state NMR of plant and fungal cell walls: A critical review.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Solid-state NMR of plant and fungal cell walls: A critical review.
Solid State Nucl Magn Reson. 2020 Mar 26;107:101660
Authors: Zhao W, Fernando LD, Kirui A, Deligey F, Wang T
Abstract
The cell walls of plants and microbes are a central source for bio-renewable energy and the major targets of antibiotics and antifungal...
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04-07-2020 07:35 PM
[NMR paper] Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana: Insights from Solid-State NMR.
Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana: Insights from Solid-State NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana: Insights from Solid-State NMR.
Biomacromolecules. 2017 Sep 11;18(9):2937-2950
Authors: Phyo P,...
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05-04-2018 03:33 PM
Multidimensional solid-state NMR spectroscopy of plant cell walls #DNPNMR
From The DNP-NMR Blog:
Multidimensional solid-state NMR spectroscopy of plant cell walls #DNPNMR
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Wang, T., P. Phyo, and M. Hong, Multidimensional solid-state NMR spectroscopy of plant cell walls. Solid State Nuclear Magnetic Resonance, 2016. 78: p. 56-63.
http://www.sciencedirect.com/science/article/pii/S0926204016300595
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11-19-2016 08:35 PM
[NMR paper] Multidimensional solid-state NMR spectroscopy of plant cell walls.
Multidimensional solid-state NMR spectroscopy of plant cell walls.
Multidimensional solid-state NMR spectroscopy of plant cell walls.
Solid State Nucl Magn Reson. 2016 Aug 13;78:56-63
Authors: Wang T, Phyo P, Hong M
Abstract
Plant biomass has become an important source of bio-renewable energy in modern society. The molecular structure of plant cell walls is difficult to characterize by most atomic-resolution techniques due to the insoluble and disordered nature of the cell wall. Solid-state NMR (SSNMR) spectroscopy is uniquely...
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08-24-2016 04:39 PM
[NMR paper] Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations.
Biomacromolecules. 2016 May 18;
Authors: Wang T, Yang H, Kubicki JD, Hong M
Abstract
The native cellulose of bacterial, algal, and animal origins has been well studied structurally using X-ray and neutron...
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05-19-2016 10:13 AM
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
From The DNP-NMR Blog:
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
Wang, T., et al., Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls. Proc Natl Acad Sci U S A, 2013. 110(41): p. 16444-9.
http://www.ncbi.nlm.nih.gov/pubmed/24065828
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11-26-2013 01:19 AM
[NMR paper] Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Related Articles Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.
Proc Natl Acad Sci U S A. 2013 Sep 24;
Authors: Wang T, Park YB, Caporini MA, Rosay M, Zhong L, Cosgrove DJ, Hong M
Abstract
Structure determination of protein binding to noncrystalline macromolecular assemblies such as plant cell walls (CWs) poses a significant structural biology challenge. CWs are loosened during growth by expansin proteins,...
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09-27-2013 03:28 AM
High resolution 13C-detected solid-state NMR spectroscopy of a deuterated protein
High resolution 13C-detected solid-state NMR spectroscopy of a deuterated protein
Abstract High resolution 13C-detected solid-state NMR spectra of the deuterated beta-1 immunoglobulin binding domain of the protein G (GB1) have been collected to show that all 15N, 13Cā?², 13CĪ± and 13CĪ² sites are resolved in 13Cā??13C and 15Nā??13C spectra, with significant improvement in T 2 relaxation times and resolution at high magnetic field (750 MHz). The comparison of echo T 2 values between deuterated and protonated GB1 at various spinning rates and under different decoupling schemes indicates...
Probing Cell-Surface Interactions in Fungal Cell Walls by High-Resolution 1H-detected Solid-State NMR Spectroscopy
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