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Default Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-

Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-saturation.

Related Articles Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-saturation.

Biochemistry. 2005 Jan 18;44(2):518-23

Authors: Morgan WD, Frenkiel TA, Lock MJ, Grainger M, Holder AA

We have applied NMR cross-saturation with TROSY detection to the problem of precisely mapping conformational epitopes on complete protein antigen molecules. We have investigated complexes of the Fab fragments of two antibodies that have parasite inhibitory activity, bound to the important malaria vaccine candidate antigen, Plasmodium falciparum MSP1(19). The results indicate remarkable overlap between these epitopes for inhibitory antibodies, and will provide a basis for theoretical modeling of the antibody-antigen interface.

PMID: 15641776 [PubMed - indexed for MEDLINE]



Source: PubMed
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