Positively-Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.
 

Positively-Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.

Related Articles Positively-Charged Tags Impede Protein Mobility in Cells as Quantified by 19F NMR.

J Phys Chem B. 2019 May 01;:

Authors: Ye Y, Wu Q, Zheng W, Jiang B, Pielak GJ, Liu M, Li C

Abstract
Proteins are often tagged for visualization or delivery in the "sea" of other macromolecules in cells but how tags affect protein mobility remains poorly understood. Here, we employ 19F in-cell NMR to quantify the mobility of proteins with charged tags in Escherichia coli cells and Xenopus laevis oocytes. We find that the transient charge-charge interactions between the tag and cellular components affect protein mobility. More specifically, positively-charged tags impede protein mobility.


PMID: 31042382 [PubMed - as supplied by publisher]



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