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Default Polysorbate 80 and Histidine Quantitative Analysis by NMR in the presence of Virus-like Particles

Polysorbate 80 and Histidine Quantitative Analysis by NMR in the presence of Virus-like Particles

Polysorbate-80 (PS80) and histidine are common excipients in vaccine and therapeutic protein formulation. A simple quantitative NMR method to measure both PS80 and histidine in human papillomavirus (HPV) virus-like particle (VLP) vaccine for aqueous and alum-containing samples is described. The new NMR method is compared to current colorimetric methods for PS80 and RP HPLC for histidine. The new NMR method is comparable to current assays with an advantage of a simpler sample treatment for PS80....

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