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Default Parallel acquisition of multi-dimensional spectra in protein NMR

Parallel acquisition of multi-dimensional spectra in protein NMR


Abstract We introduce the use of multiple receivers applied in parallel for simultaneously recording multi-dimensional data sets of proteins in a single experiment. The utility of the approach is established through the introduction of the 2D 15N,1HN||13CO HSQC experiment in which a pair of two-dimensional 15N,1HN and 15N,13CO spectra are recorded. The methodology is further extended to higher dimensionality via the 3D 1HN||13CO HNCA in which a pair of data sets recording 13Cα,15N,1HN and 13Cα,15N,13CO chemical shifts are acquired. With the anticipated increases in probe sensitivity it is expected that multiple receiver experiments will become an important approach for efficient recording of NMR data.
  • Content Type Journal Article
  • Category Communication
  • Pages 1-7
  • DOI 10.1007/s10858-012-9646-1
  • Authors
    • ?riks Kup?e, Agilent Technologies, 6 Mead Road, Yarnton, Oxford, OX5 1QU UK
    • Lewis E. Kay, Departments of Molecular Genetics, Biochemistry and Chemistry, The University of Toronto, Toronto, ON M5S 1A8, Canada

Source: Journal of Biomolecular NMR
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