The orthosteric agonist-binding pocket in the prototypic class B G-protein-coupled secretin receptor.
 

The orthosteric agonist-binding pocket in the prototypic class B G-protein-coupled secretin receptor.

http://www.ncbi.nlm.nih.gov/corehtml..._pm120_VOR.jpg Related Articles The orthosteric agonist-binding pocket in the prototypic class B G-protein-coupled secretin receptor.

Biochem Soc Trans. 2013 Feb 1;41(1):154-8

Authors: Miller LJ, Dong M

Abstract
Class B GPCRs (G-protein-coupled receptors) share heptahelical topology and G-protein binding with other superfamily members, yet have unique structures and modes of activation. Natural ligands for these receptors are moderate-length peptides with C-terminal ?-helices. NMR and crystal structures of the peptide-bound disulfide-bonded receptor N-terminal domains demonstrate that these helices occupy a conserved groove; however, the details of this interaction vary from one receptor to another. In this review, we focus on the prototypic secretin receptor and use extensive intrinsic photoaffinity labelling, structure-activity series, alanine-replacement mutagenesis and fluorescence analysis to define the molecular basis for this interaction. Additionally, experimental validation of predictions coming from in silico molecular modelling has provided a basis for enhancement of binding affinity. Such insights will be useful in the rational development of drugs acting at this important group of targets.


PMID: 23356276 [PubMed - in process]



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