Related ArticlesOrdered phosphorylation events in two independent cascades of the PTEN C-tail revealed by NMR.
J Am Chem Soc. 2012 Dec 19;134(50):20533-43
Authors: Cordier F, Chaffotte A, Terrien E, Préhaud C, Theillet FX, Delepierre M, Lafon M, Buc H, Wolff N
Abstract
PTEN phosphatase is a tumor suppressor controlling notably cell growth, proliferation and survival. The multisite phosphorylation of the PTEN C-terminal tail regulates PTEN activity and intracellular trafficking. The dynamical nature of such regulatory events represents a crucial dimension for timing cellular decisions. Here we show that NMR spectroscopy allows reporting on the order and kinetics of clustered multisite phosphorylation events. We first unambiguously identify in vitro seven bona fide sites modified by CK2 and GSK3? kinases and two new sites on the PTEN C-terminal tail. Then, monitoring the formation of transient intermediate phosphorylated states, we determine the sequence of these reactions and calculate their apparent rate constants. Finally, we assess the dynamic formation of these phosphorylation events induced by endogenous kinases directly in extracts of human neuroblastoma cells. Taken together, our data indicate that two cascades of events controlled by CK2 and GSK3? occur independently on two clusters of sites (S380-S385 and S361-S370) and that in each cluster the reactions follow an ordered model with a distributive kinetic mechanism. Besides emphasizing the ability of NMR to quantitatively and dynamically follow post-translational modifications, these results bring a temporal dimension on the establishment of PTEN phosphorylation cascades.
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03-09-2013 11:56 PM
[NMR paper] Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA.
Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA.
Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA.
J Biomol NMR. 2013 Mar 2;
Authors: Smet-Nocca C, Launay H, Wieruszeski JM, Lippens G, Landrieu I
Abstract
The Pin1 protein plays a critical role in the functional regulation of the hyperphosphorylated neuronal Tau protein in Alzheimer's disease and is by itself regulated by phosphorylation. We have...
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03-05-2013 03:25 PM
Ordered PhosphorylationEvents in Two IndependentCascades of the PTEN C-tail Revealed by NMR
Ordered PhosphorylationEvents in Two IndependentCascades of the PTEN C-tail Revealed by NMR
Florence Cordier, Alain Chaffotte, Elouan Terrien, Christophe Pre?haud, Franc?ois-Xavier Theillet, Muriel Delepierre, Monique Lafon, Henri Buc and Nicolas Wolff
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja310214g/aop/images/medium/ja-2012-10214g_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja310214g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/CgOsmhhExM8
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12-08-2012 02:48 AM
[Stan NMR blog] NMR, MRI and ESR Events Update
NMR, MRI and ESR Events Update
A major update of planned MR events from now up to the end of 2013
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03-26-2012 07:34 PM
[Stan NMR blog] NMR, MRI and ESR Events Update
NMR, MRI and ESR Events Update
A major update of planned MR events from now up to the end of 2013
Source: Stan blog library
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10-06-2011 09:01 AM
NMR events at CSC 2011
NMR events at CSC 2011
Dear colleagues,
The 94th Canadian Chemistry Conference and Exhibition (CSC 2011) "Chemistry and Health" will be held in Montréal, Québec from June 5-9, 2011, as a joint effort of the Canadian Society for Chemistry and the four Montréal Universities, with the Université de Montréal Chemistry Department as the host. The CSC is the largest annual national event for chemical professionals attracting close to 2500 participants each year.
Canada is very dynamic in Nuclear Magnetic Resonance spectroscopy and to pursue the tradition, a solid-state NMR workshop as well...
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01-27-2011 04:31 AM
[Stan NMR blog] A tsunami of MR events next January
A tsunami of MR events next January
Next year's activity in Magnetic Resonance meetings is starting in a grand way
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Ordered phosphorylation events in two independent cascades of the PTEN C-tail revealed by NMR.
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