BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 04-01-2018, 03:32 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Amyloid Structure of High-order Assembly of Leucine-Rich Amelogenin Revealed by Solid-state NMR.

Amyloid Structure of High-order Assembly of Leucine-Rich Amelogenin Revealed by Solid-state NMR.

Related Articles Amyloid Structure of High-order Assembly of Leucine-Rich Amelogenin Revealed by Solid-state NMR.

J Struct Biol. 2018 Mar 28;:

Authors: Ma CW, Zhang J, Dong XQ, Lu JX

Abstract
High-order assemblies of amelogenin, the major protein in enamel protein matrix, are believed to act as the template for enamel mineral formation. The Leucine-rich amelogenin (LRAP) is a natural splice-variant of amelogenin, a functional protein in vivo, containing conserved domains of amelogenin. In this work, we showed LRAP aggregates hierarchically into assemblies with various sizes including scattered beads, beads-on-a-string and gel-like precipitations in the presence of both calcium and phosphate ions. Solid-state NMR combined with X-ray diffraction and microscopic techniques, was applied to give a picture of LRAP self-assemblies at the atomic level. Our results, for the first time, confirmed LRAP assemblies with different sizes all contained a consistent rigid segment with ?-sheet secondary structure (residues 12-27) and the ?-sheet segment would further assemble into amyloid-like structures.


PMID: 29604451 [PubMed - as supplied by publisher]



More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] High Pressure NMR and SAXS Reveals How Capping Modulates Folding Cooperativity of the pp32 Leucine Rich Repeat Protein.
High Pressure NMR and SAXS Reveals How Capping Modulates Folding Cooperativity of the pp32 Leucine Rich Repeat Protein. High Pressure NMR and SAXS Reveals How Capping Modulates Folding Cooperativity of the pp32 Leucine Rich Repeat Protein. J Mol Biol. 2018 Mar 12;: Authors: Zhang Y, Berghaus M, Klein S, Jenkins K, Zhang S, McCallum SA, Morgan J, Winter R, Barrick D, Royer CA Abstract Many repeat proteins contain capping motifs, which serve to shield the hydrophobic core from solvent and maintain structural integrity. While the...
nmrlearner Journal club 0 03-17-2018 12:12 PM
High Pressure NMR and SAXS Reveals How Capping Modulates Folding Cooperativity of the pp32 Leucine Rich Repeat Protein
High Pressure NMR and SAXS Reveals How Capping Modulates Folding Cooperativity of the pp32 Leucine Rich Repeat Protein Publication date: Available online 13 March 2018 Source:Journal of Molecular Biology</br> Author(s): Yi Zhang, Melanie Berghaus, Sean Klein, Kelly Jenkins, Siwen Zhang, Scott A. McCallum, Joel Morgan, Roland Winter, Doug Barrick, Catherine A. Royer</br> Many repeat proteins contain capping motifs, which serve to shield the hydrophobic core from solvent and maintain structural integrity. While the role of capping motifs in...
nmrlearner Journal club 0 03-14-2018 10:02 AM
Cavities and Cooperativity in the Folding of the Leucine Rich Repeat Protein PP32: A Pressure-Jump Fluorescence and High Pressure NMR Study
Cavities and Cooperativity in the Folding of the Leucine Rich Repeat Protein PP32: A Pressure-Jump Fluorescence and High Pressure NMR Study Publication date: 3 February 2017 Source:Biophysical Journal, Volume 112, Issue 3, Supplement 1</br> Author(s): Kelly A. Jenkins, Martin Fossat, Thuy Dao, Yi Zhang, Zackery White, Doug Barrick, Catherine A. Royer</br> </br></br> </br></br> More...
nmrlearner Journal club 0 02-03-2017 09:55 PM
Structural Changes Associated with Transthyretin Misfoldingand Amyloid Formation Revealed by Solution and Solid-State NMR
Structural Changes Associated with Transthyretin Misfoldingand Amyloid Formation Revealed by Solution and Solid-State NMR http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00164/20160323/images/medium/bi-2016-00164v_0004.gif Biochemistry DOI: 10.1021/acs.biochem.6b00164 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/YkaD3mNFFNA More...
nmrlearner Journal club 0 03-24-2016 04:18 AM
[NMR paper] Structural Changes Associated with Transthyretin Misfolding and Amyloid Formation Revealed by Solution and Solid-State NMR.
Structural Changes Associated with Transthyretin Misfolding and Amyloid Formation Revealed by Solution and Solid-State NMR. Structural Changes Associated with Transthyretin Misfolding and Amyloid Formation Revealed by Solution and Solid-State NMR. Biochemistry. 2016 Mar 21; Authors: Lim KH, Dasari AK, Hung I, Gan Z, Kelly JW, Wemmer DE Abstract Elucidation of structural changes involved in protein misfolding and amyloid formation is crucial for unraveling the molecular basis of amyloid formation. Here we report structural...
nmrlearner Journal club 0 03-22-2016 01:46 PM
Structural Insight into an Alzheimer’s Brain-Derived Spherical Assembly of Amyloid ? by Solid-State NMR
Structural Insight into an Alzheimer’s Brain-Derived Spherical Assembly of Amyloid ? by Solid-State NMR Sudhakar Parthasarathy, Masafumi Inoue, Yiling Xiao, Yoshitaka Matsumura, Yo-ichi Nabeshima, Minako Hoshi and Yoshitaka Ishii http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b03373/20150518/images/medium/ja-2015-03373k_0004.gif Journal of the American Chemical Society DOI: 10.1021/jacs.5b03373 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/IQmUqF-Pq1k
nmrlearner Journal club 0 05-19-2015 09:10 AM
[NMR paper] Structural Insight into an Alzheimer's Brain-Derived Spherical Assembly of Amyloid ? by Solid-state NMR.
Structural Insight into an Alzheimer's Brain-Derived Spherical Assembly of Amyloid ? by Solid-state NMR. Related Articles Structural Insight into an Alzheimer's Brain-Derived Spherical Assembly of Amyloid ? by Solid-state NMR. J Am Chem Soc. 2015 May 4; Authors: Parthasarathy S, Inoue M, Xiao Y, Matsumura Y, Nabeshima YI, Hoshi M, Ishii Y Abstract Accumulating evidence suggests that various neurodegenerative diseases, including Alzheimer's disease (AD), are linked to cytotoxic diffusible aggregates of amyloid proteins, which are...
nmrlearner Journal club 0 05-06-2015 11:59 AM
Self-Assembly of Flexible?-Strands into ImmobileAmyloid-Like ?-Sheets in MembranesAs Revealed by Solid-State 19F NMR
Self-Assembly of Flexible?-Strands into ImmobileAmyloid-Like ?-Sheets in MembranesAs Revealed by Solid-State 19F NMR Parvesh Wadhwani, Erik Strandberg, Nico Heidenreich, Jochen Bu?rck, Susanne Fangha?nel and Anne S. Ulrich http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja301328f/aop/images/medium/ja-2012-01328f_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja301328f http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/dpJwN953qQE
nmrlearner Journal club 0 04-06-2012 01:23 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 11:03 PM.


Map