We report a microfluidic approach for one-step fabrication of polyelectrolyte microcapsules in aqueous conditions. Using two immiscible aqueous polymer solutions, we generate transient water-in-water-in-water double emulsion droplets and use them as templates to fabricate polyelectrolyte microcapsules. The capsule shell is formed by the complexation of oppositely charged polyelectrolytes at the immiscible interface. We find that attractive electrostatic interactions can significantly prolong the release of charged molecules. Moreover, we demonstrate the application of these microcapsules in encapsulation and release of proteins without impairing their biological activities. Our platform should benefit a wide range of applications that require encapsulation and sustained release of molecules in aqueous environments.Polyelectrolyte (PE) capsules: Using two immiscible aqueous solutions, dextran and PEG, transient double emulsions were created in a microfluidic system and used as templates to fabricate PE capsules. The capsule shell was formed by complexation of oppositely charged PEs, PE+ and PE-, at the interface of the two aqueous phases. This platform enables encapsulation and release of proteins without impairing their activity.
Public Release: 6-May-2015 Magic spin put on protein to tease out its 1st ... - EurekAlert (press release)
Public Release: 6-May-2015 Magic spin put on protein to tease out its 1st ... - EurekAlert (press release)
http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcT6bb2xShL8rCe4MjWU6XWHQs-DSrX4qBh_OrgUUBWs7TPY9jMID-zp4ClJoaS2VKgzbsaNxZXu
EurekAlert (press release)
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Public Release: 6-May-2015 Magic spin put on protein to tease out its 1st ...
EurekAlert (press release)
... the collaborative group of researchers used a method called Magic Angle Spinning Solid-State NMR Spectroscopy to determine the order in which the components of a protein, called...
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05-07-2015 12:59 AM
[NMR paper] NMR studies of DNA microcapsules prepared using sonochemical methods.
NMR studies of DNA microcapsules prepared using sonochemical methods.
NMR studies of DNA microcapsules prepared using sonochemical methods.
Phys Chem Chem Phys. 2014 Dec 3;
Authors: Reytblat I, Keinan-Adamsky K, Chill JH, Gottlieb HE, Gedanken A, Goobes G
Abstract
DNA molecules were recently converted using ultrasonic irradiation into microcapsules that can trap hydrophobic molecules in aqueous solution. These DNA microcapsules are capable of penetrating prokaryotic and eukaryotic cells, delivering drugs and transferring genetic...
nmrlearner
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12-04-2014 05:36 PM
[NMR paper] A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
Available online 5 March 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
</br>
Rapid freeze quench electron paramagnetic resonance (RFQ)-EPR is a method for trapping short lived intermediates in chemical reactions and subjecting them to EPR spectroscopy investigation for their characterization. Two (or more) reacting components are mixed at room temperature and after some delay the mixture is sprayed into a cold trap and transferred into...
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03-06-2013 04:33 PM
[Question from NMRWiki Q&A forum] NMR integral of Hydrophobically modified polyelectrolyte
NMR integral of Hydrophobically modified polyelectrolyte
Hi there, I have a polyelectrolyte polyethylenimine (PEI) grafted with DSPE-PEG (lipid-polyethylene glycol). I have some problem in calculating the grafting density. I do that by the integral of the two polymers.
It does not dissolve (not a clear solution) in CHCl3, CH2Cl2, MeOH, DMF. OK in water (clear after heating/sonication) and good in DMSO. But the peak of DMSO (2.5) overlap with the peak of PEI (2.4-3.3).
In water, the peak of the lipid is very bad and it is not "right" (coz it is with PEG and the ratios between PEG and...
nmrlearner
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04-25-2012 05:25 AM
[NMR paper] NMR study of a membrane protein in detergent-free aqueous solution.
NMR study of a membrane protein in detergent-free aqueous solution.
Related Articles NMR study of a membrane protein in detergent-free aqueous solution.
Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8893-8
Authors: Zoonens M, Catoire LJ, Giusti F, Popot JL
One of the major obstacles to membrane protein (MP) structural studies is the destabilizing effect of detergents. Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep MPs water-soluble under mild conditions. In the present work, we have explored the...
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11-25-2010 08:21 PM
[NMR paper] Fabrication of homogeneously cross-linked, functional alginate microcapsules validate
Fabrication of homogeneously cross-linked, functional alginate microcapsules validated by NMR-, CLSM- and AFM-imaging.
Related Articles Fabrication of homogeneously cross-linked, functional alginate microcapsules validated by NMR-, CLSM- and AFM-imaging.
Biomaterials. 2003 May;24(12):2083-96
Authors: Zimmermann H, Hillgärtner M, Manz B, Feilen P, Brunnenmeier F, Leinfelder U, Weber M, Cramer H, Schneider S, Hendrich C, Volke F, Zimmermann U
Cross-linked alginate microcapsules of sufficient mechanical strength can immunoisolate cells for the...
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11-24-2010 09:01 PM
[NMR paper] The polyelectrolyte behavior of actin filaments: a 25Mg NMR study.
The polyelectrolyte behavior of actin filaments: a 25Mg NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles The polyelectrolyte behavior of actin filaments: a 25Mg NMR study.
Biochemistry. 1999 Jun 1;38(22):7219-26
Authors: Xian W, Tang JX, Janmey PA, Braunlin WH
Under physiological conditions, filamentous actin (F-actin) is a polyanionic protein filament. Key features of the behavior of F-actin are shared with other well-characterized polyelectrolytes, in particular, duplex DNA....
One-Step Microfluidic Fabrication of Polyelectrolyte Microcapsules in Aqueous Conditions for Protein Release
Linear Mode
