Off-resonance (13)C-(2)H REDOR NMR for site-resolved studies of molecular motion
 

Off-resonance (13)C-(2)H REDOR NMR for site-resolved studies of molecular motion

We introduce a ^(13)C-²H Rotational Echo DOuble Resonance (REDOR) technique that uses the difference between on-resonance and off-resonance ²H irradiation to detect dynamic segments in deuterated molecules. By selectively inverting specific regions of the ²H magic-angle spinning (MAS) sideband manifold to recouple some of the deuterons to nearby carbons, we distinguish dynamic and rigid residues in 1D and 2D ^(13)C spectra. We demonstrate this approach on deuterated GB1, H/D exchanged GB1, and...

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