Related ArticlesObservation of reorientationally hindered water in biological tissue using triple quantum filtered 17O-NMR.
Biochim Biophys Acta. 1995 Jun 9;1244(2-3):253-8
Authors: Flesche CW, Gruwel ML, Deussen A, Schrader J
Water dynamics in aqueous biopolymer solutions often display a two-phase character, resembling water-water and water-protein interactions. Rotationally hindered water molecules in crowded protein environments display triple exponential magnetic relaxation out of the extreme narrowing limit. Because water-protein interactions retard the water dynamics, H2O(17) magnetization passes through a NMR multiple quantum coherence filter, allowing the visualization of reorientationally hindered water without the disturbing resonance of the bulk. In vitro experiments performed on selected biological materials (lens, vitreous body and serum albumin solutions) clearly demonstrate the potential of this technique.
NMR-based metabolomics of mammalian cell and tissue cultures
NMR-based metabolomics of mammalian cell and tissue cultures
Abstract NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a quantum mechanical total line shape analysis were utilized to quantify 30 metabolites such as amino acids, Krebs cycle intermediates, activated sugars, cofactors, and others in both media and cell extracts. The impact of bioreactor scale and addition of...
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NMR-based metabolomics of mammalian cell and tissue cultures.
NMR-based metabolomics of mammalian cell and tissue cultures.
NMR-based metabolomics of mammalian cell and tissue cultures.
J Biomol NMR. 2011 Mar 4;
Authors: Aranibar N, Borys M, Mackin NA, Ly V, Abu-Absi N, Abu-Absi S, Niemitz M, Schilling B, Li ZJ, Brock B, Russell RJ, Tymiak A, Reily MD
NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a...
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[NMR paper] First observation of solution structures of bradykinin-penta-O-galloyl-D-glucopyranos
First observation of solution structures of bradykinin-penta-O-galloyl-D-glucopyranose complexes as determined by NMR and simulated annealing.
Related Articles First observation of solution structures of bradykinin-penta-O-galloyl-D-glucopyranose complexes as determined by NMR and simulated annealing.
Biochim Biophys Acta. 2002 Jun 6;1571(2):89-101
Authors: Vergé S, Richard T, Moreau S, Nurich A, Merillon JM, Vercauteren J, Monti JP
Polyphenols (tannins) are known for their high propensity to precipitate proteins. They bind most strongly to...
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[NMR paper] NMR triple-quantum filtered relaxation analysis of 17O-water in insulin solutions: an
NMR triple-quantum filtered relaxation analysis of 17O-water in insulin solutions: an insight into the aggregation of insulin and the properties of its bound water.
Related Articles NMR triple-quantum filtered relaxation analysis of 17O-water in insulin solutions: an insight into the aggregation of insulin and the properties of its bound water.
Biophys Chem. 1998 Mar 9;70(3):231-9
Authors: Torres AM, Grieve SM, Kuchel PW
Transverse triple-quantum filtered NMR spectroscopy (TTQF) of 17O-water was used to study the properties of water in insulin...
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[NMR paper] Strong and weak binding of water to proteins studied by NMR triple-quantum filtered r
Strong and weak binding of water to proteins studied by NMR triple-quantum filtered relaxation spectroscopy of (17)O-water.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Strong and weak binding of water to proteins studied by NMR triple-quantum filtered relaxation spectroscopy of (17)O-water.
Biophys Chem. 1997 Sep 1;67(1-3):187-98
Authors: Torres AM, Grieve SM, Chapman BE, Kuchel PW
The triple-quantum filtered (TQF) spin-echo signal of (17)O-water, in the presence...
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[NMR paper] Proton NMR studies on ischemic rat brain tissue.
Proton NMR studies on ischemic rat brain tissue.
Related Articles Proton NMR studies on ischemic rat brain tissue.
Magn Reson Med. 1992 May;25(1):78-84
Authors: Iwama T, Yamada H, Andoh T, Sakai N, Era S, Sogami M, Kuwata K, Watari H
The spin-lattice relaxation time (T1) of water protons and the cross-relaxation time (TIS) between irradiated protein protons and observed water protons were measured in order to study water-macromolecular interactions in ischemic rat brain tissues. Tissues were obtained by bilateral common carotid artery...
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08-21-2010 11:41 PM
[NMR paper] Characterisation by triple-quantum filtered 17O-NMR of water molecules buried in lyso
Characterisation by triple-quantum filtered 17O-NMR of water molecules buried in lysozyme and trapped in a lysozyme-inhibitor complex.
Related Articles Characterisation by triple-quantum filtered 17O-NMR of water molecules buried in lysozyme and trapped in a lysozyme-inhibitor complex.
Biophys Chem. 1999 Mar 29;77(2-3):111-21
Authors: Baguet E, Hennebert N
Triple-quantum filtering NMR sequences were used to study the multiexponential relaxation behaviour of H2 17O in the presence of hen egg white lysozyme. By this means, the fraction and the...
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[NMR paper] Observation of a new nonfluorescent malondialdehyde-acetaldehyde-protein adduct by 13
Observation of a new nonfluorescent malondialdehyde-acetaldehyde-protein adduct by 13C NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Observation of a new nonfluorescent malondialdehyde-acetaldehyde-protein adduct by 13C NMR spectroscopy.
Chem Res Toxicol. 1999 Jan;12(1):100-5
Authors: Kearley ML, Patel A, Chien J, Tuma DJ
It has been shown that malondialdehyde (MDA) and acetaldehyde react with proteins via the epsilon-amino group of a lysine residue to yield hybrid...
Observation of reorientationally hindered water in biological tissue using triple qua
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