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Default Observation of acetyl phosphate formation in mammalian mitochondria using real-time in-organelle NMR metabolomics [Biochemistry]

Observation of acetyl phosphate formation in mammalian mitochondria using real-time in-organelle NMR metabolomics [Biochemistry]

Wen Jun Xu, He Wen, Han Sun Kim, Yoon-Joo Ko, Seung-Mo Dong, In-Sun Park, Jong In Yook, Sunghyouk Park...
Date: 2018-04-17

Recent studies point out the link between altered mitochondrial metabolism and cancer, and detailed understanding of mitochondrial metabolism requires real-time detection of its metabolites. Employing heteronuclear 2D NMR spectroscopy and 13C3-pyruvate, we propose in-organelle metabolomics that allows for the monitoring of mitochondrial metabolic changes in real time. The approach identified... Read More


PNAS:
Number: 16
Volume: 115
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