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-   -   [NMR paper] NMR study of non-structural proteins-part III: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Chikungunya virus (CHIKV). (http://www.bionmr.com/forum/journal-club-9/nmr-study-non-structural-proteins-part-iii-1-h-13-c-15-n-backbone-side-chain-resonance-assignment-macro-domain-chikungunya-virus-chikv-25034/)

nmrlearner 09-07-2017 10:42 PM

NMR study of non-structural proteins-part III: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Chikungunya virus (CHIKV).
 
NMR study of non-structural proteins-part III: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Chikungunya virus (CHIKV).

http://www.bionmr.com//www.ncbi.nlm....ringerlink.gif Related Articles NMR study of non-structural proteins-part III: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Chikungunya virus (CHIKV).

Biomol NMR Assign. 2017 Sep 05;:

Authors: Lykouras MV, Tsika AC, Lichière J, Papageorgiou N, Coutard B, Bentrop D, Spyroulias GA

Abstract
Macro domains are conserved protein domains found in eukaryotic organisms, bacteria, and archaea as well as in certain viruses. They consist of 130-190 amino acids and can bind ADP-ribose. Although the exact role of these domains is not fully understood, the conserved binding affinity for ADP-ribose indicates that this ligand is important for the function of the domain. Such a macro domain is also present in the non-structural protein 3 (nsP3) of Chikungunya Alphavirus (CHIKV) and consists of 160 amino acids. In this study we describe the high yield expression of the macro domain from CHIKV and its preliminary structural analysis via solution NMR spectroscopy. The macro domain seems to be folded in solution and an almost complete backbone assignment was achieved. In addition, the ?/?/? sandwich topology with 4 ?-helices and 6 ?-strands was predicted by TALOS+.


PMID: 28875416 [PubMed - as supplied by publisher]



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