NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by t
 

NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Z? domains of yatapoxvirus E3L.

Related Articles NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Z? domains of yatapoxvirus E3L.

FEBS Lett. 2010 Oct 8;

Authors: Lee EH, Seo YJ, Ahn HC, Kang YM, Kim HE, Lee YM, Choi BS, Lee JH

The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have double-stranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZ?(E3L)) bound to Z-DNA revealed that the overall structure of yabZ?(E3L) and its interaction with Z-DNA are very similar to those of hZ?(ADAR1). Here we have performed NMR hydrogen exchange experiments on the complexes between yabZ?(E3L) and d(CGCGCG)(2) with a variety of protein-to-DNA molar ratios. This study revealed that yabZ?(E3L) could efficiently change the B-form helix of the CG6 to left-handed Z-DNA via the active-mono B-Z transition pathway like hZ?(ADAR1).

PMID: 20937275 [PubMed - as supplied by publisher]



Source: PubMed