Related ArticlesNMR studies of the solution properties of recombinant murine interleukin-6.
Biochim Biophys Acta. 1995 Jun 12;1249(2):189-203
Authors: Morton CJ, Bai H, Zhang JG, Hammacher A, Norton RS, Simpson RJ, Mabbutt BC
The effects of solvent, pH and temperature on the 1H-NMR spectra of recombinant murine interleukin-6 (IL-6) are described. Assignments made from two-dimensional homonuclear spectra are presented for resonances of the fifteen aromatic amino-acid side chains. A time-dependent loss of intensity was observed for all resonances in the spectrum of IL-6, probably as a result of aggregation. This aggregation is markedly temperature-dependent. The pKa values of the four histidine residues in murine IL-6 has been measured; one has a value of 5.5, approx. one pH unit less than the value exhibited by the other three. Analysis of the NOESY spectra has allowed a preliminary characterisation of the nature of interactions among the aromatic side chains within the protein fold. 1H and 15N resonances of residues Thr-4 to Val-21 are assigned from three-dimensional 1H-15N correlated spectroscopy, and evidence is presented for these residues comprising a mobile N-terminal tail with little ordered structure. An N-terminal mutant lacking the first 22 residues of the murine IL-6 sequence and known to possess full biological activity was also examined and shown to have essentially retained the tertiary fold of the native molecule.
[NMR paper] Direct determination of the interleukin-6 binding epitope of the interleukin-6 recept
Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy.
Related Articles Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy.
J Biol Chem. 2004 Jan 2;279(1):571-6
Authors: Schwantner A, Dingley AJ, Ozbek S, Rose-John S, Grötzinger J
All cytokines belonging to the interleukin-6 (IL-6)-type family of cytokines utilize receptors that have a modular build of several immunoglobulin-like and fibronectin type III-like domains. Characteristic...
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[NMR paper] Solution structure of the recombinant oxidized rabbit uteroglobin using homonuclear a
Solution structure of the recombinant oxidized rabbit uteroglobin using homonuclear and heteronuclear multidimensional NMR.
Related Articles Solution structure of the recombinant oxidized rabbit uteroglobin using homonuclear and heteronuclear multidimensional NMR.
Eur J Biochem. 1998 Dec 1;258(2):521-32
Authors: Winkelmann R, Geschwindner S, Haun M, Rüterjans H
Rabbit uteroglobin (rab-UG) is a 16-kDa homodimeric secretory protein with potent anti-inflammatory/immunomodulatory properties. Its physiological role is still unclear, although it was...
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[NMR paper] Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine
Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine leukemia virus (MoMLV) capsid protein major-homology-region peptide analogs by NMR spectroscopy.
Related Articles Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine leukemia virus (MoMLV) capsid protein major-homology-region peptide analogs by NMR spectroscopy.
Eur J Biochem. 1998 Oct 1;257(1):69-77
Authors: Clish CB, Peyton DH, Barklis E
The capsid domain of retroviral Gag proteins possesses a single highly conserved...
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[NMR paper] NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231
NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231).
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231).
FEBS Lett. 1997 Aug 18;413(2):282-8
Authors: Riek R, Hornemann S, Wider G, Glockshuber R, Wüthrich K
The recombinant murine prion protein, mPrP(23-231), was expressed in E. coli with uniform 15N-labeling. NMR experiments showed that the previously...
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[NMR paper] Structure of recombinant human parathyroid hormone in solution using multidimensional
Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy.
Related Articles Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy.
Biol Chem Hoppe Seyler. 1996 Mar;377(3):175-86
Authors: Gronwald W, Schomburg D, Harder MP, Mayer H, Paulsen J, Wingender E, Wray V
The solution structure of human parathyroid hormone, in the form of recombinant prolyl-hPTH(1-84), has been investigated by multidimensional NMR spectroscopy under conditions (aqueous...
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[NMR paper] NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Compariso
NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
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Growth Factors. 1994;11(4):271-6
Authors: Maurer T, Smith DK, Owczarek CM, Layton MJ, Zhang JG, Nicola NA, Norton RS
Leukaemia inhibitory factor (LIF) is a polyfunctional cytokine active on many cell types. We present here 1H NMR studies on the solution properties and stability of MH35, a chimera of murine and human LIF...
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[NMR paper] NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Compariso
NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
Related Articles NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
Growth Factors. 1994;11(4):271-6
Authors: Maurer T, Smith DK, Owczarek CM, Layton MJ, Zhang JG, Nicola NA, Norton RS
Leukaemia inhibitory factor (LIF) is a polyfunctional cytokine active on many cell types. We present here 1H NMR studies on the solution properties and stability of MH35, a chimera of murine and human LIF...
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[NMR paper] Solution structure of murine epidermal growth factor determined by NMR spectroscopy a
Solution structure of murine epidermal growth factor determined by NMR spectroscopy and refined by energy minimization with restraints.
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Biochemistry. 1992 Jan 14;31(1):236-49
Authors: Montelione GT, Wüthrich K, Burgess AW, Nice EC, Wagner G, Gibson KD, Scheraga HA
The solution structure of murine epidermal growth factor (mEGF) at pH 3.1 and a temperature of 28 degrees C has been determined...
NMR studies of the solution properties of recombinant murine interleukin-6.
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