NMR studies of novel inhibitors bound to farnesyl-protein transferase.
NMR studies of novel inhibitors bound to farnesyl-protein transferase.
http://www.ncbi.nlm.nih.gov/corehtml...REE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml...pubmed-pmc.gif Related Articles NMR studies of novel inhibitors bound to farnesyl-protein transferase. Protein Sci. 1995 Apr;4(4):681-8 Authors: Koblan KS, Culberson JC, Desolms SJ, Giuliani EA, Mosser SD, Omer CA, Pitzenberger SM, Bogusky MJ Farnesyl-protein transferase (FPTase) catalyzes the posttranslational farnesylation of the cysteine residue located in the carboxyl-terminal tetrapeptide of the Ras oncoprotein. Prenylation of this residue is essential for the membrane association and cell-transforming activities of ras. Inhibitors of FPTase have been demonstrated to inhibit ras-dependent cell transformation and thus represent a potential therapeutic strategy for the treatment of human cancers. The FPTase-bound conformation of a tetrapeptide inhibitor, CVWM, and a novel pseudopeptide inhibitor, L-739,787, have been determined by NMR spectroscopy. Distance constraints were derived from two-dimensional transferred nuclear Overhauser effect experiments. Ligand competition experiments identified the NOEs that originate from the active-site conformation. Structures were calculated with the combination of distance geometry and restrained energy minimization. Both peptide backbones are shown to adopt nonideal reverse-turn conformations most closely approximating a type III beta-turn. These results provide a basis for understanding the spatial arrangements necessary for inhibitor binding and selectivity and may aid in the design of therapeutic agents. PMID: 7613466 [PubMed - indexed for MEDLINE] Source: PubMed |
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