NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases.
 

NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases.

Related Articles NMR studies of the non-haem Fe(II) and 2-oxoglutarate-dependent oxygenases.

J Inorg Biochem. 2017 Sep 04;:

Authors: Mbenza NM, Vadakkedath PG, McGillivray DJ, Leung IKH

Abstract
The non-haem Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenases belong to a superfamily of structurally-related enzymes that play important biological roles in plants, microorganisms and animals. Structural, mechanistic and functional studies of 2OG oxygenases require efficient and effective biophysical tools. Nuclear magnetic resonance (NMR) spectroscopy is a useful tool to study this enzyme superfamily. It has been applied to obtain information about enzyme kinetics, identify and characterise 2OG oxygenase-catalysed oxidation products, elucidate the catalytic mechanism, monitor ligand binding and study protein dynamics. This review summarises the types of information that NMR spectroscopy can provide in the studies of 2OG oxygenases, highlights the advantages of the technique and describes its drawbacks.


PMID: 28893416 [PubMed - as supplied by publisher]



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