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Default NMR studies of an FK-506 analog, [U-13C]ascomycin, bound to FK-506-binding protein.

NMR studies of an FK-506 analog, [U-13C]ascomycin, bound to FK-506-binding protein.

Related Articles NMR studies of an FK-506 analog, [U-13C]ascomycin, bound to FK-506-binding protein.

J Med Chem. 1992 Jun 26;35(13):2467-73

Authors: Petros AM, Gemmecker G, Neri P, Olejniczak ET, Nettesheim D, Xu RX, Gubbins EG, Smith H, Fesik SW

Multidimensional, heteronuclear NMR methods were used to determine the complete 1H and 13C resonance assignments for [U-13C]ascomycin bound to recombinant FKBP, including stereospecific assignment of all 22 methylene protons. The conformation of ascomycin was then determined from an analysis of NOEs observed in a 13C-edited 3D HMQC-NOESY spectrum of the [U-13C]ascomycin/FKBP. This structure is found to be quite different from the solution structure of the two forms of uncomplexed FK-506. However, it is very similar to the X-ray crystal structure of FK-506 bound to FKBP, rms deviation = 0.56 A. The methods used for resonance assignment and structure calculation are presented in detail. Furthermore, FKBP/ascomycin NOEs are reported which help define the structure of the ascomycin binding pocket. This structural information obtained in solution was compared to the recently described X-ray crystal structure of the FKBP/FK-506 complex.

PMID: 1377749 [PubMed - indexed for MEDLINE]



Source: PubMed
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