Related ArticlesNMR structure of the LCCL domain and implications for DFNA9 deafness disorder.
EMBO J. 2001 Oct 1;20(19):5347-53
Authors: Liepinsh E, Trexler M, Kaikkonen A, Weigelt J, Bányai L, Patthy L, Otting G
The LCCL domain is a recently discovered, conserved protein module named after its presence in Limulus factor C, cochlear protein Coch-5b2 and late gestation lung protein Lgl1. The LCCL domain plays a key role in the autosomal dominant human deafness disorder DFNA9. Here we report the nuclear magnetic resonance (NMR) structure of the LCCL domain from human Coch-5b2, where dominant mutations leading to DFNA9 deafness disorder have been identified. The fold is novel. Four of the five known DFNA9 mutations are shown to involve at least partially solvent-exposed residues. Except for the Trp91Arg mutant, expression of these four LCCL mutants resulted in misfolded proteins. These results suggest that Trp91 participates in the interaction with a binding partner. The unexpected sensitivity of the fold with respect to mutations of solvent-accessible residues might be attributed to interference with the folding pathway of this disulfide-containing domain.
Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for Structure and Dynamics
Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for Structure and Dynamics
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 5 January 2012</br>
Bernd*Reif</br>
http://www.sciencedirect.com/cache/MiamiImageURL/1-s2.0-S1090780711005969-fx1.sml</br></br></br>
Source: Journal of Magnetic Resonance
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NMR structure of CaBP1 in a Ca(2+) -bound closed state: Implications for target recognition.
NMR structure of CaBP1 in a Ca(2+) -bound closed state: Implications for target recognition.
NMR structure of CaBP1 in a Ca(2+) -bound closed state: Implications for target recognition.
Protein Sci. 2011 May 23;
Authors: Park S, Li C, Ames JB
Calcium binding protein 1 (CaBP1), a neuron-specific member of the calmodulin (CaM) superfamily, regulates the Ca(2+) dependent activity of inositol 1,4,5-triphosphate receptors (InsP3Rs) and various voltage-gated Ca(2+) channels. Here we present the NMR structure of full-length CaBP1 with Ca(2+) bound at the...
[NMR paper] NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima: implications for 216 homologous DUF59 proteins.
NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima: implications for 216 homologous DUF59 proteins.
Related Articles NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima: implications for 216 homologous DUF59 proteins.
Protein Sci. 2005 Nov;14(11):2880-6
Authors: Almeida MS, Herrmann T, Peti W, Wilson IA, Wüthrich K
The NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima represents an alpha/beta-topology formed by the regular secondary structures...
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12-01-2010 06:56 PM
[NMR paper] NMR structure of bacterial ribosomal protein l20: implications for ribosome assembly
NMR structure of bacterial ribosomal protein l20: implications for ribosome assembly and translational control.
Related Articles NMR structure of bacterial ribosomal protein l20: implications for ribosome assembly and translational control.
J Mol Biol. 2002 Oct 11;323(1):143-51
Authors: Raibaud S, Lebars I, Guillier M, Chiaruttini C, Bontems F, Rak A, Garber M, Allemand F, Springer M, Dardel F
L20 is a specific protein of the bacterial ribosome, which is involved in the early assembly steps of the 50S subunit and in the feedback control of the...
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11-24-2010 08:58 PM
[NMR paper] NMR structure of the sterol carrier protein-2: implications for the biological role.
NMR structure of the sterol carrier protein-2: implications for the biological role.
Related Articles NMR structure of the sterol carrier protein-2: implications for the biological role.
J Mol Biol. 2000 Jan 21;295(3):595-603
Authors: García FL, Szyperski T, Dyer JH, Choinowski T, Seedorf U, Hauser H, Wüthrich K
The determination of the NMR structure of the sterol carrier protein-2 (SCP2), analysis of backbone (15)N spin relaxation parameters and NMR studies of nitroxide spin-labeled substrate binding are presented as a new basis for...
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11-18-2010 09:15 PM
NMR structure of the calponin homology domain of human IQGAP1 and its implications fo
NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode
Content Type Journal Article
DOI 10.1007/s10858-010-9434-8
Authors
Ryo Umemoto, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
Noritaka Nishida, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
Shinji Ogino, The University of Tokyo Graduate School of Pharmaceutical Sciences Hongo, Bunkyo-ku Tokyo 113-0033 Japan
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Detect protein disorder to avoid wasting NMR time
Unless disordered proteins is what you are after, you may want to check if the protein you want to study with NMR is actually disordered and most likely not a very good NMR target.
The following servers for prediction of disordered regions in proteins are available. PONDR
DisEMBL
Globplot2
DISOPRED2
PDISORDER
PredictProtein
NMR structure of the LCCL domain and implications for DFNA9 deafness disorder.
Linear Mode
