Related ArticlesNMR spectroscopy reveals the presence and association of lipids and keratin in adhesive gecko setae.
Sci Rep. 2015;5:9594
Authors: Jain D, Stark AY, Niewiarowski PH, Miyoshi T, Dhinojwala A
Abstract
Lipid and protein aggregates are one of the fundamental materials of biological systems. Examples include cell membranes, insect cuticle, vertebrate epidermis, feathers, hair and adhesive structures known as 'setae' on gecko toes. Until recently gecko setae were assumed to be composed entirely of keratin, but analysis of footprints left behind by geckos walking on surfaces revealed that setae include various kinds of lipids. However, the arrangement and molecular-level behavior of lipids and keratin in the setae is still not known. In the present study we demonstrate, for the first time, the use of Nuclear Magnetic Resonance (NMR) spectroscopy techniques to confirm the presence of lipids and investigate their association with keratin in 'pristine' sheds, or natural molts of the adhesive toe pad and non-adhesive regions of the skin. Analysis was also carried on the sheds after they were 'delipidized' to remove surface lipids. Our results show a distribution of similar lipids in both the skin and toe shed but with different dynamics at a molecular level. The present study can help us understand the gecko system both biologically and for design of synthetic adhesives, but the findings may be relevant to the characteristics of lipid-protein interactions in other biological systems.
PMID: 25902194 [PubMed - as supplied by publisher]
Journal Highlight: Rapid assessment of the illegal presence of 1,3-dimethylamylamine (DMAA) in sports nutrition and dietary supplements using 1H NMR spectroscopy
Journal Highlight: Rapid assessment of the illegal presence of 1,3-dimethylamylamine (DMAA) in sports nutrition and dietary supplements using 1H NMR spectroscopy
http://www.spectroscopynow.com/common/images/thumbnails/14898526bb5.jpgA proton NMR method was developed and validated for measuring 1,3-dimethylamylamine in sports nutrition and dietary supplements and is recommended for routine use in food testing, customs or doping control laboratories.
Read the rest at Spectroscopynow.com
nmrlearner
General
0
10-06-2014 12:37 PM
[NMR paper] Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
FEBS Lett. 2014 Apr 3;
Authors: Sommer LA, Dames SA
...
nmrlearner
Journal club
0
04-08-2014 08:02 PM
[NMR paper] Application of NMR spectroscopy in the development of a biomimetic approach for hydrophobic drug association with physical hydrogels.
Application of NMR spectroscopy in the development of a biomimetic approach for hydrophobic drug association with physical hydrogels.
Related Articles Application of NMR spectroscopy in the development of a biomimetic approach for hydrophobic drug association with physical hydrogels.
Colloids Surf B Biointerfaces. 2013 Dec 19;115C:391-399
Authors: López-Cebral R, Martin-Pastor M, Paolicelli P, Casadei MA, Seijo B, Sanchez A
Abstract
The clinical application of sparingly soluble drugs is hampered by the wide range of problems associated to...
nmrlearner
Journal club
0
02-08-2014 05:45 PM
‘q-titration’ of long-chain and short-chain lipids differentiates between structured and mobile residues of membrane proteins studied in bicelles by solution NMR spectroscopy
‘q-titration’ of long-chain and short-chain lipids differentiates between structured and mobile residues of membrane proteins studied in bicelles by solution NMR spectroscopy
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 25 October 2011</br>
Woo Sung*Son, Sang Ho*Park, Henry J.*Nothnagel, George J.*Lu, Yan*Wang, ...</br>
‘q-titration’ refers to the systematic comparison of signal intensities in solution NMR spectra of uniformlyN labeled membrane proteins solubilized in micelles and isotropic bicelles as a function of the molar ratios (q) of the...
nmrlearner
Journal club
0
10-26-2011 11:25 AM
NMR spectroscopy and surface tension measurements applied to the study of self-association of casopitant mesylate, a novel NK1 antagonist.
NMR spectroscopy and surface tension measurements applied to the study of self-association of casopitant mesylate, a novel NK1 antagonist.
NMR spectroscopy and surface tension measurements applied to the study of self-association of casopitant mesylate, a novel NK1 antagonist.
J Pharm Biomed Anal. 2011 Jan 5;54(1):48-52
Authors: Provera S, Beato S, Cimarosti Z, Turco L, Casazza A, Caivano G, Marchioro C
The aggregation behaviour of casopitant mesylate, a new NK1 antagonist drug, was investigated by means of NMR spectroscopy and surface tension...
nmrlearner
Journal club
0
01-11-2011 11:27 PM
[NMR paper] Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
Related Articles Proton NMR spectroscopy shows lipids accumulate in skeletal muscle in response to burn trauma-induced apoptosis.
FASEB J. 2005 Sep;19(11):1431-40
Authors: Astrakas LG, Goljer I, Yasuhara S, Padfield KE, Zhang Q, Gopalan S, Mindrinos MN, Dai G, Yu YM, Martyn JA, Tompkins RG, Rahme LG, Tzika AA
Burn trauma triggers hypermetabolism and muscle wasting via increased cellular protein degradation and apoptosis. Proton...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
NMR Reveals Two-Step Association of Congo Red to Amyloid ? in Low-Molecular-Weight Ag
NMR Reveals Two-Step Association of Congo Red to Amyloid ? in Low-Molecular-Weight Aggregates.
Related Articles NMR Reveals Two-Step Association of Congo Red to Amyloid ? in Low-Molecular-Weight Aggregates.
J Phys Chem B. 2010 Nov 15;
Authors: Pedersen MO, Mikkelsen K, Behrens MA, Pedersen JS, Enghild JJ, Skrydstrup T, Malmendal A, Nielsen NC
Aggregation of the Amyloid ? peptide into amyloid fibrils is closely related to development of Alzheimer's disease. Many small aromatic compounds have been found to act as inhibitors of fibril formation, and...
nmrlearner
Journal club
0
11-17-2010 05:49 PM
[NMR paper] Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: appl
Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
Related Articles Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
J Biomol NMR. 1995 Nov;6(3):321-8
Authors: Dingley AJ, Mackay JP, Chapman BE, Morris MB, Kuchel PW, Hambly BD, King GF
At the millimolar concentrations required for structural studies, NMR spectra of the calcium-binding protein myosin light chain 2 (MLC2) showed resonance line widths indicative...
NMR spectroscopy reveals the presence and association of lipids and keratin in adhesive gecko setae.
Linear Mode
