NMR solution structure of a cyanovirin homolog from wheat head blight fungus.
 

NMR solution structure of a cyanovirin homolog from wheat head blight fungus.

NMR solution structure of a cyanovirin homolog from wheat head blight fungus.

Proteins. 2011 Jan 18;

Authors: Matei E, Louis JM, Jee J, Gronenborn AM

Members of the cyanovirin-N homolog (CVNH) lectin family are found in bacteria, fungi and plants. As part of our ongoing work on CVNH structure-function studies, we determined the high-resolution NMR solution structure of the homolog from the wheat head blight disease causing ascomycetous fungus Gibberella zeae (or Fusarium graminearum), hereafter called GzCVNH. Like cyanovirin-N (CV-N), GzCVNH comprises two tandem sequence repeats and the protein sequence exhibits 30% identity with CV-N. The overall structure is similar to those of other members of the CVNH family, with the conserved pseudo-symmetric halves of the structure, domains A and B, closely resembling recently determined structures of Tuber borchii, Neurospora crassa, and Ceratopteris richardii CVNH proteins. Although GzCVNH exhibits a similar glycan recognition profile to CV-N and specifically binds to Man?(1-2)Man?, its weak carbohydrate binding affinity to only one binding site is insufficient for conferring anti-HIV activity. Proteins 2011; © 2011 Wiley-Liss, Inc.

PMID: 21365681 [PubMed - as supplied by publisher]



Source: PubMed