BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 11-24-2010, 09:16 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default NMR solution structure of attractin, a water-borne protein pheromone from the mollusk

NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica.

Related Articles NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica.

Biochemistry. 2003 Aug 26;42(33):9970-9

Authors: Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W

Water-borne protein pheromones are essential for coordination of reproductive activities in many marine organisms. In this paper, we describe the first structure of a pheromone protein from a marine organism, that of attractin (58 residues) from Aplysia californica. The NMR solution structure was determined from TOCSY, NOESY, and DQF-COSY measurements of recombinant attractin expressed in insect cells. The sequential resonance assignments were done with standard manual procedures. Approximately 90% of the 949 unambiguous NOESY cross-peaks were assigned automatically with simultaneous three-dimensional structure calculation using our NOAH/DIAMOD/FANTOM program suite. The final bundle of energy-refined structures is well-defined, with an average rmsd value to the mean structure of 0.72 +/- 0.12 A for backbone and 1.32 +/- 0.11 A for heavy atoms for amino acids 3-47. Attractin contains two antiparallel helices, made up of residues Ile9-Gln16 and I30-S36. The NMR distance constraints are consistent with the three disulfide bonds determined by mass spectroscopy (C4-C41, C13-C33, and C20-C26), where the first two could be directly determined from NOESY cross-peaks between CH beta protons of the corresponding cysteines. The second helix contains the (L/I)(29)IEECKTS(36) sequence conserved in attractins from five species of Aplysia that could interact with the receptor. The sequence and structure of this region are similar to those of the recognition helix of the Er-11 pheromone of the unicellular ciliate Euplotes raikovi, suggesting a possible common pathway for intercellular communication of these two distinct pheromone families.

PMID: 12924946 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Site-resolved measurement of water-protein interactions by solution NMR.
Site-resolved measurement of water-protein interactions by solution NMR. Site-resolved measurement of water-protein interactions by solution NMR. Nat Struct Mol Biol. 2011 Jan 2; Authors: Nucci NV, Pometun MS, Wand AJ The interactions of biological macromolecules with water are fundamental to their structure, dynamics and function. Historically, characterization of the location and residence times of hydration waters of proteins in solution has been quite difficult. Confining proteins within the nanoscale interior of a reverse micelle slows water...
nmrlearner Journal club 0 01-05-2011 09:51 PM
[NMR paper] NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiologica
NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. Related Articles NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. FEBS Lett. 2002 Nov 6;531(2):314-8 Authors: Lee D, Damberger FF, Peng G, Horst R, Güntert P, Nikonova L, Leal WS, Wüthrich K The nuclear magnetic resonance structure of the unliganded pheromone-binding protein (PBP) from Bombyx mori at pH above 6.5, BmPBP(B), consists of seven helices with residues 3-8, 16-22, 29-32, 46-59, 70-79, 84-100, and...
nmrlearner Journal club 0 11-24-2010 08:58 PM
[NMR paper] NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five
NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds. Related Articles NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds. J Mol Biol. 2001 Nov 9;313(5):923-31 Authors: Zahn R, Damberger F, Ortenzi C, Luporini P, Wüthrich K The NMR solution structure of the 51 residue pheromone Er-23 from the ciliated protozoan Euplotes raikovi (Er) was calculated with the torsion angle dynamics program DYANA from 582 nuclear Overhauser enhancement (NOE) upper...
nmrlearner Journal club 0 11-19-2010 08:44 PM
[NMR paper] The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplote
The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1996 Aug;5(8):1512-22 Authors: Luginbühl P, Wu J, Zerbe O, Ortenzi C,...
nmrlearner Journal club 0 08-22-2010 02:20 PM
[NMR paper] Native like structure and stability of apo AI in a n-propanol/water solution as deter
Native like structure and stability of apo AI in a n-propanol/water solution as determined by 13C NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Native like structure and stability of apo AI in a n-propanol/water solution as determined by 13C NMR. FEBS Lett. 1995 Mar 13;361(1):29-34 Authors: Leroy A, Lippens G, Wieruszeski JM, Parra HJ, Fruchart JC To elucidate the molecular details of the conformation of apolipoprotein AI (apo AI), we have developed an approach...
nmrlearner Journal club 0 08-22-2010 03:41 AM
[NMR paper] The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes
The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes raikovi. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1994 Sep;3(9):1527-36 Authors: Mronga S, Luginbühl P, Brown LR, Ortenzi C,...
nmrlearner Journal club 0 08-22-2010 03:29 AM
[NMR paper] The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes
The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1994 Sep;3(9):1515-26 Authors: Ottiger M, Szyperski T, Luginbühl P, Ortenzi C, Luporini P,...
nmrlearner Journal club 0 08-22-2010 03:29 AM
[NMR paper] Identification and localization of bound internal water in the solution structure of
Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy. Related Articles Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy. Biochemistry. 1990 Jun 19;29(24):5671-6 Authors: Clore GM, Bax A, Wingfield PT, Gronenborn AM ...
nmrlearner Journal club 0 08-21-2010 10:48 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 08:31 PM.


Map