NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expres
 

NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expressed in humoral hypercalcemia of malignancy.

http://www.ncbi.nlm.nih.gov/corehtml...REE_120x27.gif Related Articles NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expressed in humoral hypercalcemia of malignancy.

Eur J Biochem. 1993 Jan 15;211(1-2):205-11

Authors: Ray FR, Barden JA, Kemp BE

The structure of the biologically active N-terminal domain of human parathyroid-hormone-related protein (residues 1-34) containing an Ala substituted for a His in position 26 was studied by two-dimensional proton NMR spectroscopy. Unambiguous NMR assignments of all backbone and side-chain hydrogens were made with the aid of totally correlated spectroscopy experiments, which provided through-bond 1H-1H connectivities, and NOE spectroscopy, which provided through-space and sequential backbone connectivities. The NMR data were utilized in distance-geometry algorithms to generate a family of structures. The major structural features include two segments of alpha-helix extending from Glu4 to Lys13 and from Leu27 to Thr33, with two turns from Gln16 to Arg19 and Phe22 to His25. A salt-bridge appears likely between Arg20 and Glu30 which may be critical for holding the receptor-binding domain together.

PMID: 8425530 [PubMed - indexed for MEDLINE]



Source: PubMed