NMR restraint analysis of transforming growth factor alpha: a key component for NMR s
 

NMR restraint analysis of transforming growth factor alpha: a key component for NMR structure refinement.

Related Articles NMR restraint analysis of transforming growth factor alpha: a key component for NMR structure refinement.

Proteins. 1992 Aug;13(4):306-26

Authors: Brown FK, Hempel JC, Jeffs PW

Structures of the protein, transforming growth factor alpha (TGF-alpha), have been derived from NMR data using distance geometry and subsequent energy refinement. Analysis of the sequential NOE distance bounds using a template algorithm provides a check for consistency in the calculation of bounds, stereospecific assignment of prochiral centers, and secondary structure assignment. Application of the template algorithm to the long range NOEs found within the NMR data sets collected at pH 6.3 and pH 3.4 is used to assess the confidence levels for the accuracy of the structures obtained from modeling. The method also provides critical insight in differentiating regions of the structure that are well defined from those that are not. Use of the restraint analysis protocol is shown to be a powerful adjunct to currently used methods for the assignment of protein structures from NMR data.

PMID: 1518801 [PubMed - indexed for MEDLINE]



Source: PubMed