Related ArticlesNMR resonance assignments for a ProQ homolog from Legionella pneumophila.
Biomol NMR Assign. 2018 Jun 22;:
Authors: Immer C, Hacker C, Wöhnert J
Abstract
Regulation of gene expression on a post-transcriptional level by small non-coding regulatory RNAs (sRNAs) is very common in bacteria. sRNAs base pair with sequences in their target messenger RNAs (mRNAs) and thereby regulate translation initiation or mRNA stability. Specialized RNA-binding proteins (RBPs) facilitate these regulatory sRNA/mRNA interactions by acting as RNA chaperones. A well-known example for such an RNA chaperone which is widespread in bacteria is the Hfq protein. Recently, the ProQ/FinO protein family was identified as a new class of RNA chaperones involved in sRNA based regulation. Only a few members of this protein family have been structurally characterized so far. In particular, the structural basis for RNA-binding and recognition has not yet been established for this class of proteins. Here, we report the 1H, 13C and 15N NMR resonance assignments for a ProQ homolog (Lpp 1663) from the gram-negative pathogenic bacterium Legionella pneumophila which will facilitate further structural and dynamic studies of this protein and its interaction with RNA targets.
PMID: 29934867 [PubMed - as supplied by publisher]
[NMR paper] Solution NMR Structures of Active Pyrenophora tritici-repentis ToxB and its Inactive Homolog Reveal Potential Determinants of Toxin Activity.
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J Biol Chem. 2014 Jul 26;
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Abstract
Ptr ToxB (ToxB) is a proteinaceous host-selective toxin produced by Pyrenophora...
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Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis.
Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis.
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Biomol NMR Assign. 2011 Jul 6;
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Eukaryotic ribosome biogenesis requires the concerted action of ~200 auxiliary protein factors on the nascent ribosome. For many of these...
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NMR solution structure of a cyanovirin homolog from wheat head blight fungus.
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Proteins. 2011 Jan 18;
Authors: Matei E, Louis JM, Jee J, Gronenborn AM
Members of the cyanovirin-N homolog (CVNH) lectin family are found in bacteria, fungi and plants. As part of our ongoing work on CVNH structure-function studies, we determined the high-resolution NMR solution structure of the homolog from the wheat head blight disease causing ascomycetous fungus Gibberella zeae (or Fusarium...
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The increasing antibiotic resistance of an important human pathogen Staphylococcus aureus calls for the development of new therapeutic strategies. Staphylococcal cysteine...
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[NMR paper] The linker histone homolog Hho1p from Saccharomyces cerevisiae represents a winged he
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Hho1p is assumed to serve as a linker histone in Saccharomyces cerevisiae and, notably, it possesses two putative globular...
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Proc IEEE Comput Soc Bioinform Conf. 2003;2:197-208
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NMR resonance peak assignment is one of the key steps in solving an NMR protein structure. The assignment process links resonance peaks to individual residues of the target protein sequence, providing the prerequisite for establishing intra- and inter-residue spatial relationships between atoms. The assignment process is tedious and time-consuming,...
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J Biomol NMR. 1998 May;11(4):457-8
Authors: Veeraraghavan S, Mello CC, Lee KM, Androphy EJ, Baleja JD
NMR resonance assignments for a ProQ homolog from Legionella pneumophila.
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