Related ArticlesNMR resonance assignment and dynamics of profilin from Heimdallarchaeota.
Sci Rep. 2020 Sep 28;10(1):15867
Authors: Haq SR, Survery S, Hurtig F, Lindås AC, Chi CN
Abstract
The origin of the eukaryotic cell is an unsettled scientific question. The Asgard superphylum has emerged as a compelling target for studying eukaryogenesis due to the previously unseen diversity of eukaryotic signature proteins. However, our knowledge about these proteins is still relegated to metagenomic data and very little is known about their structural properties. Additionally, it is still unclear if these proteins are functionally homologous to their eukaryotic counterparts. Here, we expressed, purified and structurally characterized profilin from Heimdallarchaeota in the Asgard superphylum. The structural analysis shows that while this profilin possesses similar secondary structural elements as eukaryotic profilin, it contains additional secondary structural elements that could be critical for its function and an indication of divergent evolution.
[NMR paper] NMR resonance assignment and backbone dynamics of a C-terminal domain homolog of orange carotenoid protein.
NMR resonance assignment and backbone dynamics of a C-terminal domain homolog of orange carotenoid protein.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR resonance assignment and backbone dynamics of a C-terminal domain homolog of orange carotenoid protein.
Biomol NMR Assign. 2020 Sep 16;:
Authors: Maksimov EG, Laptev GY, Blokhin DS, Klochkov VV, Slonimskiy YB, Sluchanko NN, Friedrich T, Chang CF, Polshakov VI
Abstract
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[NMR paper] Self-Assembly of Human Profilin-1 Detected by CPMG NMR Spectroscopy.
Self-Assembly of Human Profilin-1 Detected by CPMG NMR Spectroscopy.
Related Articles Self-Assembly of Human Profilin-1 Detected by CPMG NMR Spectroscopy.
Biochemistry. 2017 Jan 04;:
Authors: Rennella E, Sekhar A, Kay LE
Abstract
Protein oligomerization in the cell has important implications in both health and disease and an understanding of the mechanisms by which proteins can self-associate is, therefore, of critical interest. Initial stages of the oligomerization process can be hard to detect, as they often involve the...
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[NMR paper] Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations.
Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-oup_oa.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural...
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Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Abstract One bottleneck in NMR structure determination lies in the laborious and time-consuming process of side-chain resonance and NOE assignments. Compared to the well-studied backbone resonance assignment problem, automated side-chain resonance and NOE assignments are relatively less explored. Most NOE assignment algorithms require nearly complete side-chain resonance assignments from a series of through-bond experiments such as HCCH-TOCSY or HCCCONH. Unfortunately, these TOCSY...
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[NMR paper] Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spec
Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy.
FEBS Lett. 1994 Jan 10;337(2):145-51
Authors: Archer SJ, Vinson VK, Pollard TD, Torchia DA
The multifunctional protein profilin is one of the most abundant proteins in the cytoplasm and is thought to regulate actin assembly and the...
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[NMR paper] Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spec
Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy.
FEBS Lett. 1994 Jan 10;337(2):145-51
Authors: Archer SJ, Vinson VK, Pollard TD, Torchia DA
The multifunctional protein profilin is one of the most abundant proteins in the cytoplasm and is thought to regulate actin assembly and the...
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08-22-2010 03:33 AM
[NMR paper] Relaxation study of the backbone dynamics of human profilin by two-dimensional 1H-15N
Relaxation study of the backbone dynamics of human profilin by two-dimensional 1H-15N NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Relaxation study of the backbone dynamics of human profilin by two-dimensional 1H-15N NMR.
FEBS Lett. 1993 Dec 28;336(3):457-61
Authors: Constantine KL, Friedrichs MS, Bell AJ, Lavoie TB, Mueller L, Metzler WJ
The dynamic properties of 111 backbone HN sites in uncomplexed human profilin, a protein of 139 residues, have been...
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[NMR paper] Characterization of the three-dimensional solution structure of human profilin: 1H, 1
Characterization of the three-dimensional solution structure of human profilin: 1H, 13C, and 15N NMR assignments and global folding pattern.
Related Articles Characterization of the three-dimensional solution structure of human profilin: 1H, 13C, and 15N NMR assignments and global folding pattern.
Biochemistry. 1993 Dec 21;32(50):13818-29
Authors: Metzler WJ, Constantine KL, Friedrichs MS, Bell AJ, Ernst EG, Lavoie TB, Mueller L
Human profilin is a 15-kDa protein that plays a major role in the signaling pathway leading to cytoskeletal...
NMR resonance assignment and dynamics of profilin from Heimdallarchaeota.
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