NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.
 

NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.

NMR resonance assignment of the autoimmunity protein SpaI from Bacillus subtilis ATCC 6633.

Biomol NMR Assign. 2011 Jun 5;

Authors: Christ NA, Duchardt-Ferner E, Düsterhus S, Kötter P, Entian KD, Wöhnert J

Bacillus subtilis ATCC 6633 produces the lipid II targeting lantibiotic subtilin. For self-protection these gram-positive bacteria express a cluster of four self-immunity proteins named SpaIFEG. SpaI is a 16.8*kDa lipoprotein which is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Together with the ABC-transporter SpaFEG, SpaI protects the membrane from subtilin insertion and there is evidence for a direct interaction of SpaI with subtilin. As a prerequisite for further structural studies of SpaI and the SpaI/subtilin complex we report here the full (1)H, (15)N, (13)C chemical shift assignment for a stable 14.9 kDa C-terminal fragment of SpaI.

PMID: 21643970 [PubMed - as supplied by publisher]



Source: PubMed