NMR Reporter Assays for the Quantification of Weak-Affinity Receptor-Ligand Interactions
 

NMR Reporter Assays for the Quantification of Weak-Affinity Receptor-Ligand Interactions

Biophysical methods are widely employed in academia and the pharmaceutical industry to detect and quantify weak molecular interactions. Such methods find broad application in fragment-based drug discovery (FBDD). In an FBDD campaign, a suitable affinity determination method is key to advancing a project beyond the initial screening phase. Protein-observed (PO) nuclear magnetic resonance (NMR) finds widespread use due to its ability to sensitively detect very weak interactions at residue-level...

More...