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nmrlearner 03-26-2019 03:55 PM

NMR model structure of the antimicrobial peptide maximin 3.
 
NMR model structure of the antimicrobial peptide maximin 3.

http://www.bionmr.com//www.ncbi.nlm....ringerlink.gif Related Articles NMR model structure of the antimicrobial peptide maximin 3.

Eur Biophys J. 2019 Mar;48(2):203-212

Authors: Benetti S, Timmons PB, Hewage CM

Abstract
Maximin 3 is a 27-residue-long cationic antimicrobial peptide found in the skin secretion and brain of the Chinese red-belly toad Bombina maxima. The peptide is of biological interest as it possesses anti-HIV activity, not found in the other maximin peptides, in addition to antimicrobial, antitumor and spermicidal activities. The three-dimensional structure of maximin 3 was obtained in a 50/50% water/2,2,2-trifluoroethanol-d3 mixture using two-dimensional NMR spectroscopy. Maximin 3 was found to adopt an ?-helical structure from residue G1 to A22, and a coil structure with a helical propensity in the C-terminal tail. The peptide is amphipathic, showing a clear separation between polar and hydrophobic residues. Interactions with sodium dodecyl sulfate micelles, a widely used bacterial membrane-mimicking environment, were modeled using molecular dynamics simulations. The peptide maintained an ?-helical conformation, occasionally displaying a flexibility around residues G9 and G16, which is likely responsible for the peptide's low haemolytic activity. It is found to preferentially adopt a position parallel to the micellar surface, establishing a number of hydrophobic and electrostatic interactions with it.


PMID: 30734844 [PubMed - indexed for MEDLINE]



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