NMR Methods to Study the Dynamics of SH2 Domain-Phosphopeptide Complexes
NMR Methods to Study the Dynamics of SH2 Domain-Phosphopeptide Complexes
Nuclear magnetic resonance (NMR) spectroscopy is the method of choice for studying the dynamics of biological macromolecules in solution. By exploiting the intricate interplay between the effects of protein motion (both overall rotational diffusion and internal mobility) and nuclear spin relaxation, NMR allows molecular motion to be probed at atomic resolution over a wide range of timescales, including picosecond (bond vibrations and methyl-group rotations), nanosecond (loop motions and... More... |
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