NMR methods for exploring 'dark' states in ligand binding and protein-protein interactions
 

NMR methods for exploring 'dark' states in ligand binding and protein-protein interactions

A survey, primarily based on work in the authors' laboratory during the last 10 years, is provided of recent developments in NMR studies of exchange processes involving protein-ligand and protein-protein interactions. We start with a brief overview of the theoretical background of Dark state Exchange Saturation Transfer (DEST) and lifetime line-broadening (?R(2)) NMR methodology. Some limitations of the DEST/?R(2) methodology in applications to molecular systems with intermediate molecular...

More...