Related ArticlesNMR metabolomics to study the metabolic response of human osteoblasts to non-poled and poled poly (L-lactic) acid.
Magn Reson Chem. 2019 May 06;:
Authors: Araújo R, Carneiro TJ, Marinho P, da Costa MM, Roque A, da Cruz E Silva OAB, Fernandes MH, Vilarinho PM, Gil AM
Abstract
Untargeted Nuclear Magnetic Resonance (NMR) metabolomics was employed, for the first time to our knowledge, to characterize the metabolome of Human Osteoblast (HOb) cells and extracts in the presence of non-poled or negatively poled poly-L-lactic acid (PLLA). The metabolic response of these cells to this polymer, extensively used in bone regeneration strategies, may potentially translate into useful markers indicative of in vivo biomaterial performance. We present preliminary results of multivariate and univariate analysis of NMR spectra, which have shown the complementarity of lysed cells and extracts in terms of information on cell metabolome, and unveiled that, irrespective of poling state, PLLA-grown cells seem to experience enhanced oxidative stress and activated energy metabolism, at the cost of storage lipids and glucose. Possible changes in protein and nucleic acid metabolisms were also suggested, as well as enhanced membrane biosynthesis. Therefore, the presence of PLLA seems to trigger cell catabolism and anti-oxidative protective mechanisms in HOb cells, while directing them towards cellular growth. This was not sufficient, however, to lead to a visible cell proliferation enhancement in the presence of PLLA, although a qualitative tendency for negatively-poled PLLA to be more effective in sustaining cell growth than non-poled PLLA was suggested. These preliminary results indicate the potential of NMR metabolomics in enlightening cell metabolism in response to biomaterials and their properties, justifying further studies of the fine effects of poled PLLA on these and other cells of significance in tissue regeneration strategies.
PMID: 31058384 [PubMed - as supplied by publisher]
[NMR paper] Plasma metabolomics study of the hepatoprotective effect of glycyrrhetinic acid on realgar-induced sub-chronic hepatotoxicity in mice via 1H NMR analysis.
Plasma metabolomics study of the hepatoprotective effect of glycyrrhetinic acid on realgar-induced sub-chronic hepatotoxicity in mice via 1H NMR analysis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Plasma metabolomics study of the hepatoprotective effect of glycyrrhetinic acid on realgar-induced sub-chronic hepatotoxicity in mice via 1H NMR analysis.
J Ethnopharmacol. 2017 Aug 17;208:36-43
Authors: Huo T, Fang Y, Zhang Y, Wang Y, Feng C, Yuan M, Wang S,...
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02-08-2018 04:32 PM
[NMR paper] Whole Blood Reveals More Metabolic Detail of the Human Metabolome than Serum as Measured by 1H-NMR Spectroscopy: Implications for Sepsis Metabolomics.
Whole Blood Reveals More Metabolic Detail of the Human Metabolome than Serum as Measured by 1H-NMR Spectroscopy: Implications for Sepsis Metabolomics.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pt.wkhealth.com-pt-pt-core-template-journal-lwwgateway-images-pmlogoPubMed.jpg Related Articles Whole Blood Reveals More Metabolic Detail of the Human Metabolome than Serum as Measured by 1H-NMR Spectroscopy: Implications for Sepsis Metabolomics.
Shock. 2015 Sep;44(3):200-8
Authors: Stringer KA, Younger JG, McHugh C, Yeomans L,...
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05-03-2016 01:13 PM
Using [1- C]lactic acid for hyperpolarized C MR cardiac studies
From The DNP-NMR Blog:
Using lactic acid for hyperpolarized C MR cardiac studies
Chen, A.P., et al., Using lactic acid for hyperpolarized C MR cardiac studies. Magn Reson Med, 2014: p. n/a-n/a.
http://www.ncbi.nlm.nih.gov/pubmed/25046652
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01-08-2015 01:02 AM
[NMR paper] NMR metabolomics of human lung tumours reveals distinct metabolic signatures for adenocarcinoma and squamous cell carcinoma.
NMR metabolomics of human lung tumours reveals distinct metabolic signatures for adenocarcinoma and squamous cell carcinoma.
Related Articles NMR metabolomics of human lung tumours reveals distinct metabolic signatures for adenocarcinoma and squamous cell carcinoma.
Carcinogenesis. 2014 Nov 3;
Authors: Rocha CM, Barros AS, Goodfellow BJ, Carreira IM, Gomes A, Sousa V, Bernardo J, Carvalho L, Gil AM, Duarte IF
Abstract
Lung tumour subtyping, particularly the distinction between adenocarcinoma (AdC) and squamous cell carcinoma...
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11-05-2014 04:04 PM
[NMR paper] 1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles 1H NMR study on the short- and long-term impact of two training programs of sprint running on the metabolic fingerprint of human serum.
J Proteome Res. 2013 Jan 4;12(1):470-80
Authors: Pechlivanis A, Kostidis S, Saraslanidis P, Petridou A, Tsalis G, Veselkov K, Mikros E, Mougios V, Theodoridis GA...
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06-08-2013 02:18 PM
1H NMR Metabolomics Study of Human Urine
1H NMR Metabolomics Study of Human Urine
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1H NMR Metabolomics Study of Human Urine
A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
From:BioStatsXtra
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07-30-2012 07:42 AM
NMR metabolic and physiological markers of therapeutic response.
NMR metabolic and physiological markers of therapeutic response.
NMR metabolic and physiological markers of therapeutic response.
Adv Exp Med Biol. 2011;701:129-35
Authors: Lee SC, Poptani H, Delikatny EJ, Pickup S, Nelson DS, Schuster SJ, Nasta SD, Svoboda J, Goldstein SC, Wallace SG, Loevner LA, Mellon EA, Reddy R, Glickson JD
Identification of reliable metabolic and physiological NMR detectable markers for prediction and early detection of therapeutic response is essential to enabling NMR guided individualized therapy for cancer. Because...
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08-17-2011 01:33 PM
[NMR paper] Lactic acid and protein interactions: implications for the NMR visibility of lactate
Lactic acid and protein interactions: implications for the NMR visibility of lactate in biological systems.
Related Articles Lactic acid and protein interactions: implications for the NMR visibility of lactate in biological systems.
Biochim Biophys Acta. 1999 Jan 4;1426(1):177-84
Authors: Chatham JC, Forder JR
The addition of bovine serum albumin (BSA) to a solution of lactate and alanine resulted in the disappearance of the 1H-NMR resonances from lactate but not alanine. As temperature is increased lactate becomes increasingly NMR visible and...
NMR metabolomics to study the metabolic response of human osteoblasts to non-poled and poled poly (L-lactic) acid.
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