Publication date: November 2014 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 83
Author(s): Lorraine Brennan
Metabolomics is the study of metabolites present in biological samples such as biofluids, tissue/cellular extracts and culture media. Measurement of these metabolites is achieved through use of analytical techniques such as NMR and mass spectrometry coupled to liquid chromatography. Combining metabolomic data with multivariate data analysis tools allows the elucidation of alterations in metabolic pathways under different physiological conditions. Applications of NMR-based metabolomics have grown in recent years and it is now widely used across a number of disciplines. The present review gives an overview of the developments in the key steps involved in an NMR-based metabolomics study. Furthermore, there will be a particular emphasis on the use of NMR-based metabolomics in nutrition research. Graphical abstract
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
From The DNP-NMR Blog:
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
Le, D., et al., Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers. Macromolecules, 2014: p. 140613123939001.
http://pubs.acs.org/doi/abs/10.1021/ma500788n
nmrlearner
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06-18-2014 06:09 PM
[NMR paper] Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination.
Methods Mol Biol. 2014;1091:215-27
Authors: Yang Y, Ramelot TA, Ni S, McCarrick RM, Kennedy MA
Abstract
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06-10-2014 04:49 PM
Protocols and applications of cellular metabolomics in safety studies using precision-cut tissue slices and carbon 13 NMR.
Protocols and applications of cellular metabolomics in safety studies using precision-cut tissue slices and carbon 13 NMR.
Protocols and applications of cellular metabolomics in safety studies using precision-cut tissue slices and carbon 13 NMR.
Methods Mol Biol. 2011;691:205-25
Authors: Baverel G, Renault S, Faiz H, El Hage M, Gauthier C, Duplany A, Ferrier B, Martin G
Numerous xenobiotics are toxic to human and animal cells by interacting with their metabolism, but the precise metabolic step affected and the biochemical mechanism behind such a...
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02-08-2011 06:28 PM
NMR Sample Preparation from NESG Wiki
NMR Sample Preparation from NMR2.0 Wiki
http://www.nmr2.buffalo.edu/nesg.wiki/NMR_Sample_Preparation
originally posted by Main.Gaohua.Liu (11 Feb 2007)
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markber
BioNMR Wiki
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08-16-2010 04:04 AM
Workshop on Biomolecular NMR Sample Preparation - Wolfson College, Oxford
A wide range of talks on various aspects of biomolecular NMR sample preparation and protein expression can be found on the Wolfson College Website:
http://www.bioch.ox.ac.uk/nmrwolfson/
Topics include:
In vitro expression and selective protein labelling
Expression and selective modification of small peptides
Sample types for biological solid state NMR
Making nanocrystals, bicelles and nanodiscs for structural studies
Rex
Educational web pages
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09-04-2008 01:17 PM
Sample preparation
Sample preparation Samples
The Samples program is designed to collect and store some essential information pertaining to the samples used to record the spectra.
Residual Dipolar Couplings
Bicelle preparation notes from Avanti
Instructions for bicelle preparation from Avanti
NMR-based metabolomics: From sample preparation to applications in nutrition research
Highlights
Linear Mode
