[NMR paper] NMR metabolomics highlights sphingosine kinase-1 as a new molecular switch in the orchestration of aberrant metabolic phenotype in cancer cells.
Related ArticlesNMR metabolomics highlights sphingosine kinase-1 as a new molecular switch in the orchestration of aberrant metabolic phenotype in cancer cells.
Mol Oncol. 2017 May;11(5):517-533
Authors: Bernacchioni C, Ghini V, Cencetti F, Japtok L, Donati C, Bruni P, Turano P
Abstract
Strong experimental evidence in animal and cellular models supports a pivotal role of sphingosine kinase-1 (SK1) in oncogenesis. In many human cancers, SK1 levels are upregulated and these increases are linked to poor prognosis in patients. Here, by employing untargeted NMR-based metabolomic profiling combined with functional validations, we report the crucial role of SK1 in the metabolic shift known as the Warburg effect in A2780 ovarian cancer cells. Indeed, expression of SK1 induced a high glycolytic rate, characterized by increased levels of lactate along with increased expression of the proton/monocarboxylate symporter MCT1, and decreased oxidative metabolism, associated with the accumulation of intermediates of the tricarboxylic acid cycle and reduction in CO2 production. Additionally, SK1-expressing cells displayed a significant increase in glucose uptake paralleled by GLUT3 transporter upregulation. The role of SK1 is not limited to the induction of aerobic glycolysis, affecting metabolic pathways that appear to support the biosynthesis of macromolecules. These findings highlight the role of SK1 signaling axis in cancer metabolic reprogramming, pointing out innovative strategies for cancer therapies.
[NMR paper] Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
Related Articles Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using (1)H HR-MAS NMR spectroscopy.
J Pharm Biomed Anal. 2017 Sep 12;146:324-328
Authors: Maria RM, Altei WF, Selistre-de-Araujo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic...
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09-16-2017 09:58 PM
[NMR paper] Targeted Molecular Imaging of Cancer Cells Using MS2-Based (129)Xe NMR.
Targeted Molecular Imaging of Cancer Cells Using MS2-Based (129)Xe NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Targeted Molecular Imaging of Cancer Cells Using MS2-Based (129)Xe NMR.
Bioconjug Chem. 2016 Aug 17;27(8):1796-801
Authors: Jeong K, Netirojjanakul C, Munch HK, Sun J, Finbloom JA, Wemmer DE, Pines A, Francis MB
Abstract
We have synthesized targeted, selective, and highly sensitive (129)Xe NMR nanoscale biosensors using a...
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06-20-2017 04:14 PM
[NMR paper] Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Effects of doxorubicin, cisplatin, and tamoxifen on the metabolic profile of human breast cancer MCF-7 cells as determined by 1H HR-MAS NMR.
Biochemistry. 2017 Apr 05;:
Authors: Maria RM, Altei WF, Selistre-de-Araújo HS, Colnago LA
Abstract
Doxorubicin, cisplatin, and tamoxifen are part of many chemotherapeutic regimens. However, there are limited studies investigating the way...
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04-06-2017 12:16 PM
[NMR paper] Changes in metabolic markers in insulin-producing ?-cells during hypoxia-induced cell death as studied by NMR metabolomics.
Changes in metabolic markers in insulin-producing ?-cells during hypoxia-induced cell death as studied by NMR metabolomics.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Changes in metabolic markers in insulin-producing ?-cells during hypoxia-induced cell death as studied by NMR metabolomics.
J Proteome Res. 2013 Aug 2;12(8):3738-45
Authors: Tian L, Kim HS, Kim H, Jin X, Jung HS, Park KS, Cho KW, Park S, Moon WK
Abstract
This study was designed to investigate...
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02-19-2014 12:07 AM
[NMR paper] Quantitative 1H-NMR-metabolomics reveals extensive metabolic reprogramming and the effect of the aquaglyceroporin FPS1 in ethanol-stressed yeast cells.
Quantitative 1H-NMR-metabolomics reveals extensive metabolic reprogramming and the effect of the aquaglyceroporin FPS1 in ethanol-stressed yeast cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Quantitative 1H-NMR-metabolomics reveals extensive metabolic reprogramming and the effect of the aquaglyceroporin FPS1 in ethanol-stressed yeast cells.
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09-07-2013 09:54 PM
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...
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03-09-2011 04:19 AM
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
J Biomol NMR. 2011 Mar 4;
Authors: Weljie AM, Bondareva A, Zang P, Jirik FR
Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics...
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03-05-2011 01:02 PM
spectroscopy to fingerprint a person's metabolic phenotype
spectroscopy to fingerprint a person's metabolic phenotype
Now, NMR spectroscopy has revealed that an "omic" other than genomic could provide a unique view of each of the 6.7 billion people on earth - their metabonomic fingerprint.
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NMR metabolomics highlights sphingosine kinase-1 as a new molecular switch in the orchestration of aberrant metabolic phenotype in cancer cells.
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