BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 11-24-2010, 09:01 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,134
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default NMR investigation of the alkaline-like conformational transition of horse heart cytoc

NMR investigation of the alkaline-like conformational transition of horse heart cytochrome c in the presence of exogenous thiazole.

Related Articles NMR investigation of the alkaline-like conformational transition of horse heart cytochrome c in the presence of exogenous thiazole.

Biophys Chem. 2003 Jun 1;104(2):459-68

Authors: Yao Y, Tang W

The conformational transition of horse heart cyt c in the presence of exogenous thiazole is investigated by NMR spectroscopy. Surprisingly, besides the native form and the ligand-bound form, another species (species A) exists at neutral pH. Titration of thiazole at alkaline condition indicates that this form has Lys as the axial ligands and in fact is one of alkaline cyt c conformers. At a high concentration of thiazole, species A has a dominant population at a pH of approximately 7. It is the first time that a pure alkaline conformer of cyt c is obtained at neutral pH. The observed NOEs show that species A maintains the overall protein fold although large structural rearrangements are expected in the distal pocket. Furthermore, the different effect of imidazole, pyridine and thiazole on the alkaline transformation of cyt c is discussed.

PMID: 12878313 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] Proton NMR study of chemically modified horse heart ferricytochrome c confirms the pr
Proton NMR study of chemically modified horse heart ferricytochrome c confirms the presence of histidine and lysine-ligated conformers in 30% acetonitrile solution. Related Articles Proton NMR study of chemically modified horse heart ferricytochrome c confirms the presence of histidine and lysine-ligated conformers in 30% acetonitrile solution. J Inorg Biochem. 2003 Apr 1;94(4):381-5 Authors: Sivakolundu SG, Mabrouk PA Comparison of the 1H NMR spectra for guanidinated ferricyt c and chloro(terpyridine)platinum(II)-modified ferricyt c in 30%...
nmrlearner Journal club 0 11-24-2010 09:01 PM
[NMR paper] Protein hydration and location of water molecules in oxidized horse heart cytochrome
Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR. Related Articles Protein hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR. J Magn Reson. 2000 Nov;147(1):1-8 Authors: Bertini I, Huber JG, Luchinat C, Piccioli M The hydration properties of the oxidized form of horse heart cytochrome c have been studied by (1)H NMR spectroscopy. Two-dimensional, homonuclear ePHOGSY-NOESY experiments are used to map water-protein interactions. The detected NOEs reveal...
nmrlearner Journal club 0 11-19-2010 08:29 PM
[NMR paper] 1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hem
1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hemoglobin of a midge larva (Tokunagayusurika akamusi). Related Articles 1H NMR study of dynamics and thermodynamics of acid-alkaline transition in ferric hemoglobin of a midge larva (Tokunagayusurika akamusi). Biochim Biophys Acta. 1998 Jun 11;1385(1):89-100 Authors: Koshikawa K, Yamamoto Y, Kamimura S, Matsuoka A, Shikama K One of the components of hemoglobin from the larval hemolyph of Tokunagayusurika akamusi possesses naturally occurring substitution at the...
nmrlearner Journal club 0 11-17-2010 11:06 PM
[NMR paper] 31P-NMR analysis of congestive heart failure in the SHHF/Mcc-facp rat heart.
31P-NMR analysis of congestive heart failure in the SHHF/Mcc-facp rat heart. Related Articles 31P-NMR analysis of congestive heart failure in the SHHF/Mcc-facp rat heart. J Mol Cell Cardiol. 1998 Feb;30(2):235-41 Authors: Michael O'Donnell J, Narayan P, Bailey MQ, Abduljalil AM, Altschuld RA, McCune SA, Robitaille PM 31P-NMR was used to monitor myocardial bioenergetics in compensated and failing SHHF/MCC-fa(cp) (SHF) rat hearts. The SHHF/Mcc-fa(cp) (spontaneous hypertension and heart failure) rat is a relatively new genetic model in which...
nmrlearner Journal club 0 11-17-2010 11:06 PM
[NMR paper] An NMR investigation of the conformational effect of nitroxide spin labels on Ala-ric
An NMR investigation of the conformational effect of nitroxide spin labels on Ala-rich helical peptides. Related Articles An NMR investigation of the conformational effect of nitroxide spin labels on Ala-rich helical peptides. J Magn Reson. 1998 Apr;131(2):248-53 Authors: Bolin KA, Hanson P, Wright SJ, Millhauser GL Nitroxide spin labels, in conjunction with electron spin resonance (ESR) experiments, are extensively employed to probe the structure and dynamics of biomolecules. One of the most ubiquitous spin labeling reagents is the...
nmrlearner Journal club 0 11-17-2010 11:06 PM
[NMR paper] Solution structure of horse heart ferricytochrome c and detection of redox-related st
Solution structure of horse heart ferricytochrome c and detection of redox-related structural changes by high-resolution 1H NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solution structure of horse heart ferricytochrome c and detection of redox-related structural changes by high-resolution 1H NMR. Biochemistry. 1996 Sep 24;35(38):12275-86 Authors: Qi PX, Beckman RA, Wand AJ A model for the solution structure of horse heart ferricytochrome c has been determined by nuclear magnetic...
nmrlearner Journal club 0 08-22-2010 02:20 PM
[NMR paper] Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR
Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR and restrained simulated annealing. Related Articles Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR and restrained simulated annealing. Biochemistry. 1994 May 31;33(21):6408-17 Authors: Qi PX, Di Stefano DL, Wand AJ A model for the solution structure of horse heart ferrocytochrome c has been determined by nuclear magnetic resonance spectroscopy combined with hybrid distance geometry-simulated annealing calculations....
nmrlearner Journal club 0 08-22-2010 03:33 AM
[NMR paper] Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR
Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR and restrained simulated annealing. Related Articles Solution structure of horse heart ferrocytochrome c determined by high-resolution NMR and restrained simulated annealing. Biochemistry. 1994 May 31;33(21):6408-17 Authors: Qi PX, Di Stefano DL, Wand AJ A model for the solution structure of horse heart ferrocytochrome c has been determined by nuclear magnetic resonance spectroscopy combined with hybrid distance geometry-simulated annealing calculations....
nmrlearner Journal club 0 08-22-2010 03:33 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 06:59 PM.


Map