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Disordered proteins:
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Default A NMR guided approach for CsrA-RNA crystallization.

A NMR guided approach for CsrA-RNA crystallization.

Related Articles A NMR guided approach for CsrA-RNA crystallization.

J Biomol NMR. 2013 Jan 29;

Authors: Koharudin LM, Boelens R, Kaptein R, Gronenborn AM

Abstract
Structure determination of protein-nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA-nucleic acid complex, guided entirely by results from nuclear magnetic resonances spectroscopy (NMR) spectroscopy. Using a construct that lacks thirteen non-essential C-terminal residues, efficient binding to DNA could be demonstrated. One CsrA dimer interacts with two DNA oligonucleotides, similar to previous findings with RNA. Furthermore, the NMR study of the CsrA-DNA complex was the basis for successfully homing in on conditions that were suitable for obtaining crystals of the CsrA-DNA complex. Our results may be useful for those cases where RNA in protein-nucleic acid complexes may be replaced by DNA.


PMID: 23359257 [PubMed - as supplied by publisher]



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