NMR paper An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation

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[NMR paper] An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation

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11-24-2010, 08:58 PM

nmrlearner

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An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation

An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation rates.

Related Articles An NMR experiment for the accurate measurement of heteronuclear spin-lock relaxation rates.

J Am Chem Soc. 2002 Sep 11;124(36):10743-53

Authors: Korzhnev DM, Skrynnikov NR, Millet O, Torchia DA, Kay LE

Rotating-frame relaxation rates, R(1)(rho), are often measured in NMR studies of protein dynamics. We show here that large systematic errors can be introduced into measured values of heteronuclear R(1)(rho) rates using schemes which are usually employed to suppress cross-correlation between dipole-dipole and CSA relaxation mechanisms. For example, in a scalar-coupled two-spin X-H spin system the use of (1)H WALTZ16 decoupling or (1)H pulses applied at regularly spaced intervals leads to a significant overestimation of heteronuclear R(1)(rho) values. The problem is studied experimentally and theoretically for (15)N-(1)H and (13)C-(1)H spin pairs, and simple schemes are described which eliminate the artifacts. The approaches suggested are essential replacements of existing methodology if accurate dynamics parameters are to be extracted from spin-lock relaxation data sets.

PMID: 12207529 [PubMed - indexed for MEDLINE]

Source: PubMed

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